Biphasic effects of FGF2 on adipogenesis

PloS One
Sooho KimDong Kun Lee

Abstract

Although stem cells from mice deficient of FGF2 have been reported to display enhanced capacity for adipogenesis, the literature using in vitro cell culture system has so far reported conflicting results on the role of FGF2 in adipogenesis. We here demonstrate that FGF2, depending on concentration, can function as either a positive or negative factor of in vitro adipogenesis by regulating activation of the ERK signaling pathway. FGF2 at concentrations lower than 2 ng/ml enhanced in vitro adipogenesis of human adipose-derived stem cells (hASCs). However, FGF2 at concentrations higher than 10 ng/ml was able to suppress adipogenesis by maintaining sustained phosphorylation of ERK and function as a dominant negative adipogenic factor toward BMP ligands. Expression levels of FGF2 in the fat tissues from high fat diet induced obese C57BL/6 mice were lower than those from normal chow diet mice, indicating that expression levels of FGF2 in the fat tissues might be in reverse correlation with the size of fat tissues. Our observation of concentration dependent biphasic effect as well as dominant negative effect of FGF2 on adipogenesis provides a mechanistic basis to understand roles of FGF2 in adipogenesis and development of fat tissues.

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Citations

Nov 22, 2018·International Wound Journal·Oliver J SmithAsh Mosahebi
Jan 29, 2019·Plastic and Reconstructive Surgery·Lauren E KokaiJ Peter Rubin
Oct 24, 2019·International Journal of Molecular Sciences·Niklas GaeblerPamela Fischer-Posovszky
Jan 15, 2021·Stem Cell Research·Takayoshi OtsukaCato T Laurencin
Jul 18, 2020·International Journal of Biological Macromolecules·Andrea De PieriDimitrios I Zeugolis
May 8, 2021·Journal of Cellular Physiology·Karolina KriaučiūnaitėAugustas Pivoriūnas
Sep 9, 2021·Proceedings of the National Academy of Sciences of the United States of America·Sebastian MathesJan Krützfeldt

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Methods Mentioned

BETA
PCR

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