Abstract
Abnormal angiogenesis underlies many pathological conditions and is critical for the growth and maintenance of various types of tumors, including hormone-dependent cancers. Since estrogens are potent carcinogens in humans and rodents, and are involved in regulating angiogenesis, this study was designed to examine the effect of estrogen on the behavior of an established bovine capillary endothelial cell line, a simple and physiologically relevant model of the capillary wall. The results demonstrate that 17beta-estradiol (E2), at different conditions, exerts both stimulatory and inhibitory effects on endothelial cell adhesion, proliferation and tube formation in vitro. Utilizing a cellular attachment assay, chronic exposure to nanomolar concentrations of E2 (i.e. 1 and 10 nM) increased endothelial cell adhesion significantly compared to vehicle treated controls. Cellular adhesion was inhibited by micromolar concentrations of E2. Cell count, PCNA immunohistochemistry and Western blot analysis demonstrated enhanced cell proliferation at low E2 concentration in estrogen-deplete medium. Inhibition of cellular proliferation was observed in both estrogen-replete and deplete medium at higher E2 concentrations (i.e. 1 and 10 microM). Fur...Continue Reading
Citations
Sep 19, 2002·The Journal of Biological Chemistry·Sang Gyu ParkSunghoon Kim
Dec 15, 2007·Endocrinology·Dongmin LiuJoseph S Dillon
May 1, 2008·Journal of Neuroendocrinology·G W AraújoS Arnold
Nov 18, 2015·Journal of Gastroenterology and Hepatology·Sharon PokNarci C Teoh
Aug 1, 2008·Expert Opinion on Drug Discovery·Sang Won LeeSunghoon Kim
Apr 15, 2000·Pancreas·S K BanerjeeD R Campbell
Dec 28, 1999·Biology of Reproduction·K HayashiD Schams
Jul 21, 2007·Endocrine Reviews·Rainer H Straub
Sep 8, 2007·Endocrinology·Javed IqbalIain J Clarke
Nov 24, 2007·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Z Khalkhali-EllisM J Hendrix
May 1, 2010·Journal of Cellular Physiology·Scott P FraserMustafa B A Djamgoz
May 25, 2011·Carcinogenesis·Robert M Bigsby, Andrea Caperell-Grant
Mar 24, 2012·Endocrine·Dorota Ptasinska-WnukHenryk Stepien
Oct 13, 2012·Human & Experimental Toxicology·E J CalabreseV Calabrese
Jun 28, 2011·Journal of Cell Communication and Signaling·Shreya BanerjeeSushanta K Banerjee
Oct 23, 2003·Endocrine Reviews·Helen E TurnerJohn A H Wass
Jun 27, 2008·Pharmacological Reviews·Virginia M Miller, Sue P Duckles
May 16, 2011·Pharmaceuticals·Jakob O StromElvar Theodorsson
Apr 16, 2020·International Journal of Environmental Research and Public Health·Paulina KurBarbara Wiszniewska
Apr 28, 2004·The American Journal of Pathology·Se-Hwa KimZorina S Galis