PMID: 8962711Nov 1, 1995Paper

Biphasic L-arginine uptake by the isolated guinea-pig heart

Experimental Physiology
M M KostićM A Rosić

Abstract

L-Arginine is the physiological substrate for the formation of nitric oxide (NO) and accounts for the biological activity of endothelium-derived relaxing factor. We have studied L-arginine transport in the heart using a rapid dual-isotope dilution technique. The time course of L-[3H]arginine uptake (extraction) by the isolated perfused guinea-pig heart was found to occur in two phases. The first phase reached a plateau in 6.6 +/- 0.6 s and lasted 8.8 +/- 0.7 s, whereas the second phase developed a plateau after 16.3 +/- 0.8 s. The first phase of maximal uptake (Umax,1) accounted for 13.4 +/- 1.4% of the total uptake and the second (Umax,2) for 32.3 +/- 1.8%. The two phases of uptake were inhibited by unlabelled L-arginine in a dose-dependent manner, which suggests that both phases are carrier mediated. The degree of inhibition of Umax,1 and Umax,2 by unlabelled L-arginine was not significantly different. Studies of the kinetics of uptake of these processes revealed an apparent Km,1 of 183 +/- 10 microM with a Vmax,1 of 50 +/- 10 nmol min-1 g-1 for the first phase and Km,2 of 167 +/- 14 microM with a Vmax,2 of 93 +/- 13 nmol min-1 g-1 for the second phase of uptake. These results suggest a similar affinity for the receptors of b...Continue Reading

Citations

Jun 5, 2014·BioMed Research International·Mirko RosicSuzana Pantovic
May 23, 2015·Circulation Journal : Official Journal of the Japanese Circulation Society·Maja ColicMirko Rosic
Feb 19, 2008·Journal of Biochemical and Biophysical Methods·Mirko RosicMilos Kojic
Dec 31, 2002·Physiological Reviews·Giovanni E MannLuis Sobrevia
Jun 19, 2021·Journal of Basic and Clinical Physiology and Pharmacology·Faten M A DiabEnas A Abdel-Hady

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