Biphasic onset of splenic apoptosis following hemorrhagic shock: critical implications for Bax, Bcl-2, and Mcl-1 proteins.

Critical Care : the Official Journal of the Critical Care Forum
Arwed HostmannSven K Tschoeke

Abstract

The innate immune response to trauma hemorrhage involves inflammatory mediators, thus promoting cellular dysfunction as well as cell death in diverse tissues. These effects ultimately bear the risk of post-traumatic complications such as organ dysfunction, multiple organ failure, or adult respiratory distress syndrome. In this study, a murine model of resuscitated hemorrhagic shock (HS) was used to determine the apoptosis in spleen as a marker of cellular injury and reduced immune functions. Male C57BL-6 mice were subjected to sham operation or resuscitated HS. At t = 0 hours, t = 24 hours, and t = 72 hours, mice were euthanized and the spleens were removed and evaluated for apoptotic changes via DNA fragmentation, caspase activities, and activation of both extrinsic and intrinsic apoptotic pathways. Spleens from untreated mice were used as control samples. HS was associated with distinct lymphocytopenia as early as t = 0 hours after hemorrhage without regaining baseline levels within the consecutive 72 hours when compared with sham and control groups. A rapid activation of splenic apoptosis in HS mice was observed at t = 0 hours and t = 72 hours after hemorrhage and predominantly confirmed by increased DNA fragmentation, eleva...Continue Reading

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Citations

Dec 25, 2009·Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine·Tareq KheirbekHasan B Alam
Oct 18, 2008·Critical Care : the Official Journal of the Critical Care Forum·Stefan U WeberFrank Stüber
Nov 6, 2009·Critical Care : the Official Journal of the Critical Care Forum·Steven M Opal, Steven P LaRosa
Oct 30, 2009·Critical Care : the Official Journal of the Critical Care Forum·Jeffery C MetzgerPaul E Pepe
Aug 29, 2012·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Gautam BandyopadhyayCarol L Miller-Graziano
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Nov 30, 2021·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Jiamin HuangBingwei Sun

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Methods Mentioned

BETA
fluorescence activated cell sorting
Assay
PCR
flow cytometry

Software Mentioned

Cell Quest
Alpha Digidoc
TreeStar
FlowJo

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