Biphasic recruitment of transcriptional repressors to the murine cytomegalovirus major immediate-early promoter during the course of infection in vivo.

Journal of Virology
Xue-feng LiuMary Hummel

Abstract

Our previous studies showed that establishment of murine cytomegalovirus (MCMV) latency in vivo is associated with repression of immediate-early gene expression, deacetylation of histones bound to the major immediate-early promoter (MIEP), changes in patterns of methylation of histones, and recruitment of cellular repressors of transcription to the MIEP. Here, we have quantitatively analyzed the kinetics of changes in viral RNA expression, DNA copy number, and recruitment of repressors and activators of transcription to viral promoters during the course of infection. Our results show that changes in viral gene expression correlate with changes in recruitment of RNA polymerase and acetylated histones to viral promoters. Binding of the transcriptional repressors histone deacetylase type 2 (HDAC2), HDAC3, YY1, CBF-1/RBP-Jk, Daxx, and CIR to the MIEP and HDACs to other promoters showed a biphasic pattern: some binding was detectable prior to activation of viral gene expression, then decreased with the onset of transcription and increased again as repression of viral gene expression occurred. Potential binding sites for CBF-1/RBP-Jk and YY1 in the MIEP and for YY1 in the M100 promoter (M100P) were identified by in silico analysis. W...Continue Reading

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Citations

Jun 26, 2012·Journal of Leukocyte Biology·Jonathan CampbellCharles H Cook
Sep 15, 2012·Journal of Cell Science·Alyshia NewhartSusan M Janicki
Mar 4, 2014·PLoS Pathogens·Franziska DağLuka Cičin-Šain
May 24, 2013·Viruses·Xue-feng LiuMary Hummel
Jan 10, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Z LiM Abecassis
May 4, 2011·Reviews in Medical Virology·Michael NevelsChristina Paulus
Oct 31, 2014·Annals of Clinical and Translational Neurology·Makiko Sakao-SuzukiIsao Kosugi
Jun 1, 2016·Frontiers in Microbiology·Anna LuganiniGiorgio Gribaudo
Apr 5, 2019·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Zheng ZhangMichael M Abecassis
Jan 31, 2020·Transplantation·Taylor A Heald-SargentMary A Hummel
Dec 19, 2017·Current Clinical Microbiology Reports·Donna Collins-McMillen, Felicia D Goodrum
Apr 17, 2020·Frontiers in Cellular and Infection Microbiology·Eleonora ForteMary Hummel

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