PMID: 19912848Feb 1, 1992Paper

Biphasic response of hippocampal dynorphin expression following recurrent limbic seizure

Molecular and Cellular Neurosciences
J D WhiteC M Gall

Abstract

The opioid peptide dynorphin (DYN) is expressed normally at high levels in dentate gyrus granule cells in hippocampus and in neurons in entorhinal and neocortex. In the present study, ribonuclease protection and in situ hybridization analyses were used to examine preproDYN mRNA expression in hippocampus and neocortex following recurrent limbic seizure induced by a unilateral electrolytic lesion of the dentate gyrus hilus. In this paradigm, electrographic seizures within hippocampus recur intermittently from 1.6 to 12 h following the hilus lesion (HL). Solution hybridization-ribonuclease protection analysis of preproDYN mRNA levels in hippocampal dentate gyrus/CA1 samples from rats sacrificed 6 and 12 h following HL revealed an approximate 6-fold increase above control values at both times. PreproDYN mRNA levels returned toward control values by 24 h post-HL, were suppressed up to 10-fold below control values at 48 and 96 h post-HL, and then returned to control levels by 10 days post-HL. In situ hybridization analyses confirmed the biphasic nature of seizure-induced changes in preproDYN expression specifically within dentate gyrus granule cells. Additionally, these latter studies demonstrated that seizures induce expression of p...Continue Reading

References

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C GallG Lynch
Dec 22, 1989·Science·J L SonnenbergT Curran
Dec 1, 1989·Molecular Endocrinology·J DouglassL Calavetta
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D W SaffenJ M Baraban
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J F McGintyF E Bloom

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Citations

Feb 1, 1994·Brain Research. Molecular Brain Research·G L YountJ D White
Nov 1, 1993·Peptides·G A OlsonA J Kastin
Jan 1, 1994·Progress in Neurobiology·G Sperk
Apr 1, 1995·Neuroscience·M B HarrisonE W Lothman

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