Biphenyl based stationary phases for improved selectivity in complex steroid assays

Journal of Pharmaceutical and Biomedical Analysis
Johanna M LindnerStefanie H Grimm

Abstract

The measurement of steroid hormones and their corticoid precursors is an important aspect in endocrinology since these analytes are biomarkers for several endocrine disorders. Over the last few years, HPLC-MS/MS has become the method of choice to analyze these compounds. There are already several methods using stationary phases modified with C18 groups. However, since these columns sometimes do not enable sufficient separation of some isobaric steroids, we investigated the potential of a different RP modification using biphenyl groups for the separation of challenging isobars such as corticosterone, 11- and 21-deoxycortisol. The aim of our work was the development of an isotope dilution UHPLC-MS/MS assay for clinical research that combines simple and effective sample preparation with a powerful MS method quantifying a broad steroid panel (aldosterone, corticosterone, cortisol, cortisone, 11-deoxycorticosterone, 11-deoxycortisol, 21-deoxycortisol, dehydroepiandrosterone, dehydroepiandrosterone sulfate, 17-OH-progesterone, progesterone, and testosterone) in human serum. After a manual protein precipitation step using zinc trifluoroacetate (ZnTFA) in methanol, the supernatants were directly injected into the UHPLC-MS system. Chrom...Continue Reading

Citations

Jun 3, 2020·Critical Reviews in Analytical Chemistry·Anna W Sobańska
Nov 24, 2020·Biomedical Chromatography : BMC·Amy GravitteStacy Brown
Dec 2, 2020·The Journal of Steroid Biochemistry and Molecular Biology·Eulalia OlestiSerge Rudaz
Nov 18, 2019·The Journal of Steroid Biochemistry and Molecular Biology·Marco MezzulloFlaminia Fanelli
Aug 16, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sridhar Chinthakindi, Kurunthachalam Kannan

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