Bipolar nanosecond electric pulses are less efficient at electropermeabilization and killing cells than monopolar pulses

Biochemical and Biophysical Research Communications
Bennett L IbeyAndrei G Pakhomov

Abstract

Multiple studies have shown that bipolar (BP) electric pulses in the microsecond range are more effective at permeabilizing cells while maintaining similar cell survival rates as compared to monopolar (MP) pulse equivalents. In this paper, we investigated whether the same advantage existed for BP nanosecond-pulsed electric fields (nsPEF) as compared to MP nsPEF. To study permeabilization effectiveness, MP or BP pulses were delivered to single Chinese hamster ovary (CHO) cells and the response of three dyes, Calcium Green-1, propidium iodide (PI), and FM1-43, was measured by confocal microscopy. Results show that BP pulses were less effective at increasing intracellular calcium concentration or PI uptake and cause less membrane reorganization (FM1-43) than MP pulses. Twenty-four hour survival was measured in three cell lines (Jurkat, U937, CHO) and over ten times more BP pulses were required to induce death as compared to MP pulses of similar magnitude and duration. Flow cytometry analysis of CHO cells after exposure (at 15 min) revealed that to achieve positive FITC-Annexin V and PI expression, ten times more BP pulses were required than MP pulses. Overall, unlike longer pulse exposures, BP nsPEF exposures proved far less effec...Continue Reading

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Citations

Jul 17, 2014·Bioelectrochemistry·Luc WasunguJustin Teissié
Apr 22, 2014·Cellular and Molecular Life Sciences : CMLS·Andrei G PakhomovBennett L Ibey
Oct 29, 2015·Bioelectromagnetics·Azadeh PeymanDamijan Miklavčič
Mar 8, 2016·Bioelectrochemistry·Tina Batista NapotnikDamijan Miklavčič
May 27, 2015·Bioelectrochemistry·Iurii SemenovAndrei G Pakhomov
Sep 13, 2014·Bioelectrochemistry·Karl H SchoenbachBennett L Ibey
Sep 3, 2016·International Journal of Radiation Biology·Wei LuGuoshan Yang
Dec 18, 2016·Cellular and Molecular Life Sciences : CMLS·Elena C GianulisAndrei G Pakhomov
Mar 19, 2016·Scientific Reports·Claudia MuratoriOlga N Pakhomova
Apr 23, 2017·Biochimica Et Biophysica Acta. Biomembranes·Caterina MerlaP Thomas Vernier
Nov 29, 2017·Scientific Reports·Chris M ValdezBennett L Ibey
Sep 14, 2017·Physics in Medicine and Biology·Borja MercadalAntoni Ivorra
Jun 14, 2020·Bioelectromagnetics·Shu XiaoStephen Beebe
Sep 7, 2017·Scientific Reports·Maura CasciolaAndrei G Pakhomov
Sep 15, 2020·Bioelectrochemistry·Rosa OrlacchioPhilippe Leveque
Jan 12, 2021·The Journal of Membrane Biology·Jody C CantuBennett L Ibey
Apr 15, 2019·Biochimica Et Biophysica Acta. Biomembranes·Esin B Sözer, P Thomas Vernier
May 8, 2018·Bioelectrochemistry·Shu XiaoSambasiva R Rajulapati
May 12, 2021·Bioelectrochemistry·Tjaša PotočnikAlenka Maček Lebar
Jun 20, 2021·Bioelectrochemistry·Tina Batista NapotnikDamijan Miklavčič
Jul 23, 2021·Advanced Science·Elise P W JenkinsGeorge G Malliaras
Aug 27, 2021·Bioelectrochemistry·Allen S KiesterJoel N Bixler
Oct 22, 2021·Proceedings of the National Academy of Sciences of the United States of America·Dengning XiaMark R Prausnitz
Oct 24, 2020·Biophysical Journal·Esin B SözerJoshua Zimmerberg

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