Bipolar properties of red seabream (Pagrus major) transforming growth factor-beta in induction of the leucocytes migration

Fish & Shellfish Immunology
Zhonghua CaiKezhi Xing

Abstract

TGF-beta is one of the pleiotropic cytokines and plays a pivotal role in immune regulation and orchestrating the subsequent healing response. Recombinant red seabream TGF-beta (rTGF-beta) mature peptide was expressed and purified under native conditions in vitro. Bio-assay showed that the rTGF-beta could significantly induce head kidney (HK) and peripheral blood (PB) leucocytes migration in a dose dependent manner, whereas the rTGF-beta suppressed HK and PB leucocyte migration when the leucocytes was activated by primed with lipopolysaccharide (LPS). Both enhancing and suppressing roles of rTGF-beta on the HKL and PBL chemotactic activity indicated that the fish TGF-beta shared the similar bipolar nature with mammalian TGF-beta. Furthermore, the results indicated that the activity of TGF-beta induction of leucocyte migration appears not to be an innate feature but function by regulation the chemokines activity. This is the first time we reported that fish TGF-beta has innate bipolar property in regulation of fish immune function.

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Citations

Mar 31, 2015·Developmental and Comparative Immunology·Xu-liang ZhanWen-sheng Li
Sep 26, 2013·Developmental and Comparative Immunology·Jacob G Schmidt, Michael E Nielsen

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