PMID: 9433804Jan 20, 1998Paper

Birth of live mice derived by in vitro fertilization with spermatozoa retrieved up to twenty-four hours after death

The Journal of Experimental Zoology
N SongsasenS P Leibo

Abstract

The objective of this study was to determine the viability and fertility of mouse spermatozoa obtained at various postmortem intervals. Male mice were euthanized, and the bodies were kept at approximately 22 degrees C for up to 24 hr. The epididymides were removed and spermatozoa were allowed to swim out into Minimum Essential Medium supplemented with bovine serum albumin. Motility of spermatozoa retrieved within 12 hr postmortem was approximately 60%, whereas motility of those obtained 6 to 12 hr later decreased significantly (P < 0.05). There appeared to be no differences in the percentages of spermatozoa with intact plasma and acrosomal membranes regardless of the time after death. After in vitro fertilization of oocytes with spermatozoa collected immediately after death or at 6, 12, 18, or 24 hr postmortem, the cleavage rates were 81%, 70%, 64%, 34%, and 19%, respectively. Once oocytes were fertilized, more than 65% developed into morulae/blastocysts. Transfer of a total of 166 embryos produced in vitro with postmortem spermatozoa resulted in the birth of 44 live pups (26.5%). Of these 44, 3 live mice were derived by transfer of 11 embryos (27.3%) produced with 24-hr postmortem spermatozoa. Histological examination of the t...Continue Reading

References

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Citations

Apr 12, 2011·Asian Journal of Andrology·Md Sharoare HossainHeriberto Rodriguez-Martinez
Mar 25, 2008·Archives of Andrology·Julián Santiago-MorenoAntonio López-Sebastián
Jan 24, 2003·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Ana J SolerJosé J Garde
Dec 6, 2011·Animal Reproduction Science·J Tamayo-CanulF Martínez-Pastor
Nov 11, 2008·Reproduction in Domestic Animals = Zuchthygiene·M R Fernández-SantosF Martínez-Pastor
Dec 14, 2011·Reproduction in Domestic Animals = Zuchthygiene·P Thuwanut, K Chatdarong
Jan 9, 2003·The Urologic Clinics of North America·Spencer Land, Lawrence S Ross
Dec 6, 2005·The Journal of Veterinary Medical Science·Tatsuya HoriToshihiko Tsutsui
Jul 7, 2009·The Journal of Veterinary Medical Science·Tatsuya HoriToshihiko Tsutsui
Sep 26, 2014·Animal : an International Journal of Animal Bioscience·D Fernández AbellaJ L Dacheux
Feb 1, 2017·Zygote : the Biology of Gametes and Early Embryos·Man-Xi JiangBao-Ping Wang
Jan 10, 2002·Genesis : the Journal of Genetics and Development·M SatoM Kimura
Sep 17, 2009·Reproduction in Domestic Animals = Zuchthygiene·K ChatdarongA Sangwornrachasup
Sep 1, 2012·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Mo GuanMartin D Fray
Jan 4, 2013·Reproduction in Domestic Animals = Zuchthygiene·C M TittarelliM A Stornelli

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