PMID: 11914121Mar 27, 2002Paper

Birth of pups after transfer of mouse embryos derived from vitrified preantral follicles

Reproduction : the Official Journal of the Society for the Study of Fertility
E C dela PeñaM Nagano

Abstract

Preantral follicles mechanically isolated from the ovaries of 12-day-old mice were exposed to 2 mol ethylene glycol l(-1) for 2 or 5 min and then to a vitrification solution containing 6 mol ethylene glycol l(-1) and 0.3 mol raffinose l(-1) for 0.5, 1.0 or 2.0 min before vitrification. The vitrified and fresh preantral follicles were treated with collagenase, and the oocyte-granulosa cell complexes (OGCs) obtained were cultured in vitro for 10 days in membrane inserts. Preantral follicles exposed to 2 mol ethylene glycol l(-1) for 5 min and then to the vitrification solution for 0.5 or 1.0 min showed the highest survival rates after warming. The follicular loss after warming was approximately 20%. After in vitro culture, the proportion of viable OGCs from the vitrified follicles was 10% lower than that of the fresh preantral follicles. There were no differences in the rates of maturation, fertilization and subsequent development to blastocysts between the oocytes derived from vitrified follicles and those derived from fresh preantral follicles; however, the developmental competence of the oocytes derived from both vitrified and fresh preantral follicles grown in vitro was lower than that of oocytes grown in vivo. One of the fiv...Continue Reading

Citations

Feb 21, 2013·Journal of Assisted Reproduction and Genetics·Marina C PeluffoMary B Zelinski
Nov 8, 2008·Nature Medicine·Mats Brännström, Milan Milenkovic
Jun 3, 2009·Reproduction, Fertility, and Development·N A CzarnyJ C Rodger
Jun 1, 2012·Biopreservation and Biobanking·Luana N SantanaRegiane R Santos
May 24, 2007·Tissue Engineering·Min XuTeresa K Woodruff
Apr 11, 2009·Human Reproduction·Victoria KerosOuti Hovatta
Mar 5, 2010·Biology of Reproduction·Susan L BarrettTeresa K Woodruff
Mar 15, 2013·The Journal of Reproduction and Development·Jiang BianCanquan Zhou
Apr 20, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·N SongsasenD E Wildt
Oct 24, 2014·Biopreservation and Biobanking·Simone Vieira CastroAna Paula Ribeiro Rodrigues
Oct 22, 2010·The Journal of Reproduction and Development·Hiroaki TaketsuruTakashi Miyano
Jun 24, 2010·Theriogenology·Rong-Mei BaoTakashi Miyano
Apr 20, 2007·The Journal of Obstetrics and Gynaecology Research·Won-Jun ChoiWon-Young Paik
Jan 12, 2007·Reproduction in Domestic Animals = Zuchthygiene·C A AmorimP B D Gonçalves
Oct 16, 2013·The Journal of Reproduction and Development·Yuji HiraoKenji Naruse
Jun 26, 2015·Reproductive Biology and Endocrinology : RB&E·Sandra SanfilippoFlorence Brugnon
Jul 7, 2011·Animal Science Journal = Nihon Chikusan Gakkaihō·Yuji Hirao
Oct 4, 2016·Systems Biology in Reproductive Medicine·Roya Hedayati KashkaTaghi Lashkarbolouki
Dec 12, 2003·Biology of Reproduction·Leen VanhoutteJohan Smitz
Jun 29, 2012·The Journal of Reproduction and Development·Yuji Hirao
Sep 5, 2003·Biology of Reproduction·Sandra CecconiBarbara Barboni
Dec 23, 2009·Reproductive Medicine and Biology·Shouko NonowakiToshitaka Horiuchi
Jun 19, 2011·Reproductive Medicine and Biology·Rong-Mei BaoTakashi Miyano
Jan 7, 2011·Systems Biology in Reproductive Medicine·Lihua LiuDouglas T Carrell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.