PMID: 8600453Mar 1, 1996Paper

Bis-intercalation of a homodimeric thiazole orange dye in DNA in symmetrical pyrimidine-pyrimidine-purine-purine oligonucleotides

Nucleic Acids Research
L F HansenJ P Jacobsen

Abstract

One- and two-dimensional 1H NMR spectroscopy were used to characterize the binding of a homodimeric thiazole orange dye, 1,1'-(4,4,8,8-tetramethyl-4,8-diazaundecamethylene)-bis-4-(3 -methyl-2,3-dihydro-(benzo- 1,3-thiazole)-2-methylidene)-quinolinium tetraiodide (TOTO), to various double-stranded DNA oligonucleotides containing symmetric (5'-pyr-pyr-pu-pu-3')2 or (5'-pu-pu-pyr-pyr-3')2 sequences. It was found that TOTO binds preferentially to oligonucleotides containing a (5'-CTAG-3')2 or a (5'-CCGG-3')2 sequence. Binding to the (5'-CCGG-3')2 sequence is less favored than to the (5'-CTAG-3')2 sequence. The complexes of TOTO with d(CGCTAGCGCTAGCG)2 (10) and d(CGCTAGCCGGCG):d(CGCCGGCTAGCG) (11) oligonucleotides, each containing two preferential binding sites, was also examined. In both cases TOTO forms mixtures of 1:1 and 1:2 dsDNA-TOTO complexes in ratios dependent on the relative amount of TOTO and the oligonucleotides in the sample. Binding of TOTO to the two oligonucleotides is sequence selective at the (5'-CTAG-3')2 and (5'-CCGG-3')2 sites. The 1H NMR spectra of both the 1:2 complexes and the three different 1:1 complexes have been assigned. A slight negative cooperativity is observed in formation of the 1:2 complexes. The r...Continue Reading

References

Nov 1, 1991·Bioconjugate Chemistry·C Bailly, J P Hénichart
Dec 1, 1989·Journal of Biomolecular Structure & Dynamics·R MarounB P Roques
May 1, 1989·Quarterly Reviews of Biophysics·X L Gao, D J Patel
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D E GilbertJ Feigon

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Citations

May 7, 2013·Environmental Science and Pollution Research International·Jie CaoLiang-Hong Guo
Dec 17, 1997·Journal of Biomolecular Structure & Dynamics·J FaridiJ P Jacobsen
Dec 2, 1998·Journal of Biomolecular Structure & Dynamics·F Johansen, J P Jacobsen
Jul 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Robert RiehnRobert H Austin
Apr 13, 2002·Photochemistry and Photobiology·Eric PrivatPaul Vigny
Jul 21, 2007·Chemistry : a European Journal·Alexandre FürstenbergEric Vauthey
Jul 7, 2012·Biochemistry·Yasumasa KimuraKengo Usui
Nov 24, 1999·Journal of Biomolecular Structure & Dynamics·C B NielsenJ P Jacobsen

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