Biscarbamate cross-linked polyethylenimine derivative with low molecular weight, low cytotoxicity, and high efficiency for gene delivery.

International Journal of Nanomedicine
Yu-Qiang WangTuo Jin

Abstract

Polyethylenimine (PEI), especially PEI 25 kDa, has been widely studied for delivery of nucleic acid drugs both in vitro and in vivo. However, it lacks degradable linkages and is too toxic for therapeutic applications. Hence, low-molecular-weight PEI has been explored as an alternative to PEI 25 kDa. To reduce cytotoxicity and increase transfection efficiency, we designed and synthesized a novel small-molecular-weight PEI derivative (PEI-Et, Mn: 1220, Mw: 2895) with ethylene biscarbamate linkages. PEI-Et carried the ability to condense plasmid DNA (pDNA) into nanoparticles. Gel retardation assay showed complete condensation of pDNA at w/w ratios that exceeded three. The particle size of polymer/pDNA complexes was between 130 nm and 180 nm and zeta potential was 5-10 mV, which were appropriate for cell endocytosis. The morphology of PEI-Et/pDNA complexes observed by atomic force microscopy (AFM) was spherically shaped with diameters of 110-190 nm. The transfection efficiency of polymer/pDNA complexes as determined with the luciferase activity assay as well as fluorescence-activated cell-sorting analysis (FACS) was higher than commercially available PEI 25 kDa and Lipofectamine 2000 in various cell lines. Also, the polymer exhibit...Continue Reading

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Citations

Oct 31, 2012·International Journal of Molecular Sciences·Paul ZarogouldisKonstantinos Zarogoulidis
Jun 20, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mohammad Ariful IslamChong-Su Cho
Jul 15, 2015·European Journal of Pharmacology·Narine A GhazaryanNaira M Ayvazyan
Sep 26, 2013·International Journal of Pharmaceutics·Hamideh ParhizMohammad Ramezani
Oct 4, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Yun-Dan SunFu-Gen Wu

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Methods Mentioned

BETA
transfection
transfecting
nuclear magnetic resonance
fluorescence
fluorescence-activated cell-sorting
protein assay
electrophoresis
atomic force microscopy
AFM
ELISA

Software Mentioned

SPSS

Related Concepts

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