Bisecting GlcNAc residues on laminin-332 down-regulate galectin-3-dependent keratinocyte motility.

The Journal of Biological Chemistry
Yoshinobu KariyaJianguo Gu

Abstract

Laminin-332 (Lm332; formerly laminin-5) is a basement membrane protein in the skin, which promotes cell motility in wound healing and cancer invasion. In a previous study, we reported that the introduction of bisecting GlcNAc into Lm332 (GnT-III-Lm332), catalyzed by N-acetylglucosaminyltransferase III (GnT-III), reduced cell migration (Kariya, Y., Kato, R., Itoh, S., Fukuda, T., Shibukawa, Y., Sanzen, N., Sekiguchi, K., Wada, Y., Kawasaki, N., and Gu, J. (2008) J. Biol. Chem. 283, 33036-33045). However, the underlying molecular mechanism by which GnT-III-Lm332 suppresses the normal biological functions of Lm332 remains to be elucidated. In this study, we show that galectin-3, which is a beta-galactoside-binding protein, strongly bound to unmodified Lm332 but not to GnT-III-Lm332 and that binding of galectin-3 was completely blocked by lactose. Exogenous galectin-3 significantly enhanced keratinocyte cell motility on control Lm332 but not on GnT-III-Lm332. A functional blocking antibody against galectin-3 inhibited Lm332-induced alpha3beta1 and alpha6beta4 integrin clustering and focal contact formation. Co-immunoprecipitation revealed that galectin-3 associated with both beta4 integrin and epidermal growth factor receptor, ther...Continue Reading

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Citations

Apr 6, 2012·Glycoconjugate Journal·Hazuki E MiwaPamela Stanley
May 9, 2012·Glycoconjugate Journal·Jianguo GuYuguang Du
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