Bisphenol A disrupts gene expression in human placental trophoblast cells

Reproductive Toxicology
Chandrew RajakumarKaiping Yang

Abstract

This study examined the effect of bisphenol A (BPA) on human placental gene expression using primary trophoblast cells as an in vitro model system. Trophoblast cells were isolated from human placentas at term, cultured and then exposed to environmentally relevant concentrations of BPA (0.1-2 μg/ml) for up to 24h, after which levels of 11β-HSD2 mRNA, protein and activity were determined by standard radiometric conversion assay, western blotting, and qRT-PCR, respectively. The mRNA levels of several other prominent placental hormones/factors were also assessed by qRT-PCR. BPA dramatically increased levels of 11β-HSD2 activity, protein and mRNA in a time- and concentration-dependent manner (> 4-fold). BPA also augmented aromatase, glucose transporter-1, CRH, and hCG mRNA levels while reducing the level of leptin mRNA. These findings demonstrate that BPA severely disrupts human placental gene expression in vitro, which suggests that exposure to BPA may contribute to altered placental function and consequent pregnancy complications.

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Citations

Oct 23, 2015·Journal of Molecular Endocrinology·Maria CerneaKaiping Yang
Jun 5, 2016·Chemosphere·Afia Usman, Masood Ahmad
Jun 14, 2017·Journal of Applied Toxicology : JAT·Agnieszka Tomza-MarciniakBogumiła Pilarczyk
Mar 21, 2018·International Journal of Molecular Sciences·Luana PaulesuCarlo Ticconi
Sep 12, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Rita S Strakovsky, Susan L Schantz
Sep 12, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Joshua F RobinsonSusan J Fisher
Feb 23, 2019·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Nancy Noyola-MartínezDavid Barrera
Feb 8, 2020·Journal of Clinical Medicine·Sophie-Christine de Aguiar GrecaEmmanouil Karteris
Mar 20, 2018·Archives of Toxicology·Jeremy GingrichAlmudena Veiga-Lopez
Feb 20, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jiude MaoCheryl S Rosenfeld
Jul 3, 2021·International Journal of Molecular Sciences·Leonardo ErminiAlessandro Rolfo

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