Bisphenol A induces apoptosis and G2-to-M arrest of ovarian granulosa cells

Biochemical and Biophysical Research Communications
Jiping XuOsamu Tsutsumi

Abstract

We investigated the impact of bisphenol A (BPA) on murine ovarian granulosa cells. Ovarian granulosa cells were cultured with 100 fM to 100 microM BPA for 24 h to 72 h. BPA decreased granulosa cell viability in a dose- and time-dependent manner. The lowest concentration that induced a significant decrease was 100 pM (89.2 +/- 4.0% of the control). TUNEL analysis demonstrated that treatment with BPA increased apoptosis of granulosa cells in a dose- and time-dependent manner. In addition, flow cytometry analyses revealed that treatment with BPA resulted in G2-to-M arrest, which was most prominent at 48 h. BPA increased the expression of Bax and concomitantly decreased the expression of Bcl2 at both protein and mRNA levels of granulosa cells. These findings suggest that low, presumably environmentally relevant doses of BPA, decrease the viability of granulosa cells by inducing apoptosis and G2-to-M arrest. Up-regulation of Bax and down-regulation of Bcl2 were suggested to be involved in this apoptotic effect.

Citations

Oct 8, 2013·Apoptosis : an International Journal on Programmed Cell Death·Indrajit ChowdhuryRoland Matthews
Feb 6, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yuan-Jie LiXiao-Lin Wu
Oct 20, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jackye PeretzJodi A Flaws
Aug 28, 2002·Congenital Anomalies·Taisen IguchiShinsuke Tanabe
Oct 20, 2010·Environmental Health : a Global Access Science Source·David CantonwineMartha Maria Téllez-Rojo
Nov 30, 2013·Human Reproduction Update·M C RichardsonN S Macklon
Jul 21, 2011·Drug and Chemical Toxicology·Chun-Chi KuoChung-Ren Jan
Jun 25, 2015·Carcinogenesis·Sabine A S LangieAndrew R Collins
Jun 14, 2017·Journal of Applied Toxicology : JAT·Agnieszka Tomza-MarciniakBogumiła Pilarczyk
Dec 8, 2016·International Journal of Experimental Pathology·Sahar El-Sayed ElswefyRehab Abdallah Hasan
Feb 17, 2017·Biology of Reproduction·Shanthi GanesanAileen F Keating
Jan 14, 2018·Reproduction in Domestic Animals = Zuchthygiene·K A CampenCmh Combelles
Sep 22, 2018·Journal of Biochemical and Molecular Toxicology·Beyza Ayazgök, Tuba Tüylü Küçükkılınç
Dec 17, 2009·International Journal of Andrology·E Mok-LinR Hauser
Feb 28, 2019·Cellular and Molecular Life Sciences : CMLS·Wei GeWei Shen
Jul 10, 2008·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Robert E ChapinSusan R Woskie
Jun 13, 2015·Biology of Reproduction·Shreya PatelJodi A Flaws
Feb 14, 2019·Environmental Science and Pollution Research International·Ahmet BilgiAhmet Birtan Boran
Jan 29, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Rodosthenis S RodosthenousRonit Machtinger
Nov 5, 2013·Expert Review of Obstetrics & Gynecology·David E CantonwineJohn D Meeker
Jun 25, 2015·Carcinogenesis·Stephanie C CaseyDean W Felsher
Dec 31, 2016·International Journal of Environmental Research and Public Health·Wei ZhouJun Zhang
Jan 17, 2013·Environmental Science and Pollution Research International·Xiaoxuan JiangWenbo Dong
Dec 17, 2016·Human Reproduction·Abdallah MansurRonit Machtinger
May 17, 2019·International Journal of Endocrinology·Ewa MatuszczakAdam Hermanowicz
Jun 25, 2015·Carcinogenesis·Patricia A ThompsonWilliam H Bisson
Jun 28, 2018·Environmental Science and Pollution Research International·Shuang MaXiuhui Zhong
Nov 1, 2020·Naunyn-Schmiedeberg's Archives of Pharmacology·Maryam Yadegari DehnaviFatemeh Zare Mehrjerdi

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