Bisphosphonates modulate the effect of macrophage-like cells on osteoblast

The International Journal of Biochemistry & Cell Biology
C E Evans

Abstract

Macrophages (MPs) are present in many tissues and have been implicated in the excessive bone resorption seen in patients with skeletal disorders. Our previous studies showed that macrophage-like cells influenced osteoblasts (OB) in co-culture, as number and activity of osteoblasts were decreased in co-cultures compared with controls. Macrophages are probable precursors of osteoblasts which have been shown to be inhibited by bisphosphonates (BPs). Bisphosphonates also modulate macrophage and osteoblasts activity. This study investigated whether addition of bisphosphonates to co-cultures of osteoblast and macrophages could reduce or block the adverse effects of macrophages on osteoblasts. The results showed that, compared to controls, fewer osteoblasts were present over time in macrophage/osteoblast co-cultures (at day 12, 15.5 x 10(4) and 8.8 x 10(4); P<0.0001) and that addition of bisphosphonates (10(-9)-10(-5)M) to the co-cultures prevented this reduction (P<0.001). Bisphosphonates also elicited an increase in numbers of osteoblast (82%) and restored alkaline phosphatase (ALP) activity, which was reduced by 15% (P approximately equal to 0.05) compared to control levels. The number of macrophages in co-cultures was reduced when...Continue Reading

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Citations

Feb 10, 2012·European Journal of Clinical Pharmacology·Nicola MaruottiFrancesco Paolo Cantatore
Jan 18, 2013·Journal of Thyroid Research·Hala Ahmadieh, Sami T Azar
Aug 7, 2003·Journal of Biomaterials Science. Polymer Edition·O Aylin GürpinarErhan Pişkin
Jul 18, 2009·Journal of Biomedical Materials Research. Part a·Babak ChehroudiDonald Maxwell Brunette
Feb 8, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Luciana F MassaVictor E Arana-Chavez
Mar 16, 2016·Beilstein Journal of Organic Chemistry·Sophie LetortFrançois Estour

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