Bistability of a coupled Aurora B kinase-phosphatase system in cell division

ELife
Anatoly V ZaytsevEkaterina L Grishchuk

Abstract

Aurora B kinase, a key regulator of cell division, localizes to specific cellular locations, but the regulatory mechanisms responsible for phosphorylation of substrates located remotely from kinase enrichment sites are unclear. Here, we provide evidence that this activity at a distance depends on both sites of high kinase concentration and the bistability of a coupled kinase-phosphatase system. We reconstitute this bistable behavior and hysteresis using purified components to reveal co-existence of distinct high and low Aurora B activity states, sustained by a two-component kinase autoactivation mechanism. Furthermore, we demonstrate these non-linear regimes in live cells using a FRET-based phosphorylation sensor, and provide a mechanistic theoretical model for spatial regulation of Aurora B phosphorylation. We propose that bistability of an Aurora B-phosphatase system underlies formation of spatial phosphorylation patterns, which are generated and spread from sites of kinase autoactivation, thereby regulating cell division.

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Citations

Apr 24, 2016·Chromosoma·Olga AfonsoHelder Maiato
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Methods Mentioned

BETA
FRET
biosensor
size-exclusion chromatography
PCR
size exclusion chromatography

Software Mentioned

Mathematica
Numerical
Prism
GraphPad
MatLab script
Parameter Estimation and Fitting Tool ' ( PEFT
Metamorph
PEFT

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