Bitumen fume-induced gene expression profile in rat lung

Toxicology and Applied Pharmacology
Laurent GateStéphane Binet

Abstract

Exposure to bitumen fumes during paving and roofing activities may represent an occupational health risk. To date, most of the studies performed on the biological effect of asphalt fumes have been done with regard to their content in carcinogenic polycyclic aromatic hydrocarbons (PAH). In order to gain an additional insight into the mechanisms of action of bitumen fumes, we studied their pulmonary effects in rodents following inhalation using the microarray technology. Fisher 344 rats were exposed for 5 days, 6 h/day to bitumen fumes generated at road paving temperature (170 degrees C) using a nose-only exposition device. With the intention of studying the early transcriptional events induced by asphalt fumes, lung tissues were collected immediately following exposure and gene expression profiles in control and exposed rats were determined by using oligonucleotide microarrays. Data analysis revealed that genes involved in lung inflammatory response as well as genes associated with PAH metabolization and detoxification were highly expressed in bitumen-exposed animals. In addition, the expression of genes related to elastase activity and its inhibition which are associated with emphysema was also modulated. More interestingly gen...Continue Reading

Citations

Jan 11, 2012·The Lancet Oncology·Beatrice Lauby-SecretanUNKNOWN WHO International Agency for Research on Cancer Monograph Working Group
Nov 6, 2010·Regulatory Toxicology and Pharmacology : RTP·Ceinwen A Schreiner
Sep 25, 2007·Mutation Research·Banalata SenDavid M DeMarini
Jul 15, 2015·Journal of Applied Toxicology : JAT·William A DewGreg G Pyle
Nov 3, 2017·Critical Reviews in Toxicology·Anthony J KriechAnthony J Riley

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