Bivalent response to long-term storage in liquid-preserved boar semen: a flow cytometric analysis

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
H HenningD Waberski

Abstract

The fertility of liquid-preserved boar semen declines during storage at 17°C, insemination trials even indicating early losses in fertilizing ability within the first 24-48 h of storage. Standard semen parameters barely reflect these changes in semen quality, and new approaches for assessment of functional changes in stored spermatozoa are needed. Capacitation, the essential prefertilization step for spermatozoa in the female genital tract, is specifically induced in vitro by bicarbonate. Therefore, we have investigated changes in responsiveness of boar spermatozoa to bicarbonate during storage. Ejaculates of 14 boars were diluted in Beltsville thawing solution, cooled to 17°C and stored for 12, 24, 72, 120, and 168 h before investigation. At each time, basic semen quality was characterized by sperm motility and viability. Subsequently, washed subsamples were incubated in variants of an in vitro fertilization (IVF) medium and assessed for kinetic changes of viability (plasma membrane integrity) and intracellular calcium concentration using flow cytometry in combination with propidium iodide and Fluo-3. By this means, it was possible to determine specific effects of bicarbonate and calcium on sperm subpopulations over incubation...Continue Reading

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Citations

Jun 20, 2014·Reproduction, Fertility, and Development·Heiko HenningDagmar Waberski
May 13, 2015·Systems Biology in Reproductive Medicine·Isabel CasasGary C Althouse
Aug 24, 2013·Reproduction in Domestic Animals = Zuchthygiene·A M Petrunkina, R A P Harrison
Jun 13, 2013·Theriogenology·Rocío Fernández-GagoFelipe Martínez-Pastor
May 31, 2018·Reproduction, Fertility, and Development·David Martin-HidalgoLuis J Garcia-Marin
Jul 15, 2015·Zygote : the Biology of Gametes and Early Embryos·Ronald Henry Fraser Hunter, Joaquín Gadea
Apr 1, 2020·Animals : an Open Access Journal From MDPI·Blanka Premrov BajukPaola Roncada
Jan 4, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Helen JäkelDagmar Waberski

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