Bivariate flow karyotyping with air-cooled lasers

Cytometry
T FreyR A Hoffman

Abstract

An experimental flow cytometer was constructed using a quartz flow cell optically coupled to a 1.22 NA lens. A pair of crossed cylindrical quartz lenses allowed multilaser excitation. Two helium-cadmium (HeCd) lasers, emitting 16 mW at 442 nm and 35 mW at 325 nm, were used to excite chromomycin A3 and Hoechst 33258 fluorescence, respectively. Bivariate flow karyotypes from normal human male chromosomes and from the Daudi cell line were obtained and were compared to those from a standard instrument using dual water-cooled lasers. The new experimental instrument exhibited comparable resolution to that from the standard instrument. In further experiments with Daudi chromosomes, the 35 mW HeCd laser was replaced with a 10 mW HeCd laser, and the system still gave good, though slightly decreased, resolution.

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Citations

Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·H J GrossD Recktenwald
May 28, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bee L Ng, Nigel P Carter
Nov 1, 1994·Developmental and Comparative Immunology·J C KunichM C Nieto
Dec 18, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bee L NgSam Thompson
Feb 26, 2010·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Khalid KhanBryan Beattie
Jun 11, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Robert A Bob Hoffman

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