BK channel beta1-subunit regulation of calcium handling and constriction in tracheal smooth muscle

American Journal of Physiology. Lung Cellular and Molecular Physiology
Iurii SemenovRobert Brenner

Abstract

The large-conductance, Ca2+-activated K+ (BK) channels are regulators of voltage-dependent Ca2+ entry in many cell types. The BK channel accessory beta1-subunit promotes channel activation in smooth muscle and is required for proper tone in the vasculature and bladder. However, although BK channels have also been implicated in airway smooth muscle function, their regulation by the beta1-subunit has not been investigated. Utilizing the gene-targeted mice for the beta1-subunit gene, we have investigated the role of the beta1-subunit in tracheal smooth muscle. In mice with the beta1-subunit-knockout allele, BK channel activity was significantly reduced in excised tracheal smooth muscle patches and spontaneous BK currents were reduced in whole tracheal smooth muscle cells. Knockout of the beta1-subunit resulted in an increase in resting Ca2+ levels and an increase in the sustained component of Ca2+ influx after cholinergic signaling. Tracheal constriction studies demonstrate that the level of constriction is the same with knockout of the beta1-subunit and BK channel block with paxillin, indicating that BK channels contribute little to airway relaxation in the absence of the beta1-subunit. Utilizing nifedipine, we found that the inc...Continue Reading

References

Mar 1, 1990·Journal of Clinical Pathology·A J RobertsonG D Smith
Jan 1, 1985·British Journal of Clinical Pharmacology·P J Barnes
Sep 1, 1995·The American Journal of Physiology·D P McCobbL Salkoff
Jun 1, 1996·Journal of Bioenergetics and Biomembranes·G J KaczorowskiM L Garcia
Jan 1, 1996·Annual Review of Physiology·M I Kotlikoff, K E Kamm
Oct 1, 1996·The American Journal of Physiology·F VogalisB Horowitz
Sep 1, 1997·The Journal of General Physiology·D H CoxR W Aldrich
Jan 21, 2000·Hypertension·W F Jackson
Sep 23, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·L TaoJ P Bourreau
Apr 27, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Yukihiro YagiHirokazu Tsubone
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Q J Li, L J Janssen
Feb 4, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yukihiro YagiHirokazu Tsubone
Jul 18, 2003·American Journal of Physiology. Cell Physiology·Brian M HagenKenton M Sanders
Aug 29, 2003·Biophysical Journal·Martin FalckePatricia Camacho
Jun 9, 2004·The Journal of Biological Chemistry·Andrea L MeredithRichard W Aldrich

❮ Previous
Next ❯

Citations

Dec 23, 2008·Cellular and Molecular Life Sciences : CMLS·J CuiU S Lee
Feb 14, 2007·The Journal of General Physiology·Olga M KovalBrad S Rothberg
Oct 29, 2008·The Journal of General Physiology·Tara-Beth Sweet, Daniel H Cox
Mar 26, 2010·Tissue Engineering. Part B, Reviews·Jeffrey A BeamishRoger E Marchant
Jul 15, 2009·Clinical and Experimental Pharmacology & Physiology·Catalina Romero-MéndezRicardo Espinosa-Tanguma
Dec 7, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Hui XuGregory D Fink
Jul 14, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shu ZhuScott A Barman
Apr 16, 2010·American Journal of Physiology. Renal Physiology·Shaohua ChangSamuel Chacko
Dec 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Xixi ChenRichard W Aldrich
Oct 26, 2013·Pflügers Archiv : European journal of physiology·Ana I Fernández-MariñoJosé M Fernández-Fernández
Aug 20, 2010·Kidney International·P Richard Grimm, Steven C Sansom
May 16, 2012·Pharmacology & Therapeutics·Alejandro M DopicoAditya K Singh
Oct 2, 2015·Journal of Neurogastroenterology and Motility·Miroslav RadulovicBing Gong
Apr 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Zhenwei SuRoderick MacKinnon
Sep 13, 2013·Channels·Gustavo F ContrerasRamón Latorre
Feb 12, 2019·Annual Review of Physiology·Vivian Gonzalez-Perez, Christopher J Lingle
Oct 21, 2010·Physiological Reviews·Henrike BerkefeldUwe Schulte
Dec 5, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sayuri NodaYuji Imaizumi
Sep 3, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Monica P GoldklangSteven O Marx
Jul 2, 2019·Journal of Medicinal Food·Rui ShiWei-Wei Su
Jun 22, 2021·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Xiaoli WangJie Tao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.