BK Channels Regulate LPS-induced CCL-2 Release from Human Pulmonary Endothelial Cells.

American Journal of Respiratory Cell and Molecular Biology
Tatiana ZyrianovaAndreas Schwingshackl

Abstract

We recently established a role for the stretch-activated two-pore-domain K+ (K2P) channel TREK-1 (K2P2.1) in inflammatory cytokine secretion using models of hyperoxia-, mechanical stretch-, and TNF-α-induced acute lung injury. We have now discovered the expression of large conductance, Ca2+-activated K+ (BK) channels in human pulmonary microvascular endothelial cells and primary human alveolar epithelial cells using semiquantitative real-time PCR, IP and Western blot, and investigated their role in inflammatory cytokine secretion using an LPS-induced acute lung injury model. As expected, LPS induced IL-6 and CCL-2 secretion from pulmonary endothelial and epithelial cells. BK activation with NS1619 decreased LPS-induced CCL-2 but not IL-6 secretion from endothelial cells and had no effect on epithelial cells, although fluorometric assays revealed that BK activation hyperpolarized the plasma membrane potential (Em) of both cell types. Interestingly, BK inhibition (Paxilline) did not alter cytokine secretion or the Em in either cell type. Furthermore, LPS treatment by itself did not affect the Em or intracellular Ca2+ concentrations. Therefore, we propose BK channel activation as a novel targeted approach to counteract LPS-induced...Continue Reading

References

Jan 4, 1994·European Journal of Pharmacology·S P OlesenJ Drejer
Dec 29, 2000·Seminars in Thrombosis and Hemostasis·A BierhausP P Nawroth
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R BlunckU Seydel
Aug 11, 2007·Histochemistry and Cell Biology·Ian De ProostDirk Adriaensen
Aug 19, 2007·Biochemical and Biophysical Research Communications·Won Sun ParkJin Han
Jan 10, 2008·Journal of Neurochemistry·Tamás GáspárDavid W Busija
Jan 30, 2008·The Journal of General Physiology·Ingrid CarvachoRamon Latorre
Dec 17, 2008·Blood·Kirill EssinRalph Kettritz
Feb 13, 2009·European Journal of Pharmacology·Wendy L ImlachJulie E Dalziel
May 12, 2010·Cold Spring Harbor Perspectives in Biology·Toby Lawrence
Sep 29, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andreas SchwingshacklChristopher M Waters
Oct 15, 2011·Journal of Cardiovascular Pharmacology·Hui XuGregory D Fink
Apr 21, 2012·The Journal of Pain : Official Journal of the American Pain Society·Birha McCannBradley E Aouizerat
Dec 19, 2012·Shock·Rachel N GomesHugo C Castro-Faria-Neto
Jan 1, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andreas SchwingshacklChristopher M Waters
Sep 13, 2013·Channels·Gustavo F ContrerasRamón Latorre
Dec 18, 2013·Bioinformatics·Andreas KrämerStuart Tugendreich
Jun 22, 2014·The Journal of Pharmacology and Experimental Therapeutics·Bernhard NauschLars C B Rønn
Aug 16, 2014·Critical Care Medicine·Andreas SchwingshacklChristopher M Waters
Oct 4, 2014·Journal of Biomolecular Screening·Mao Xiang ChenDerek Trezise
Oct 29, 2014·The Journal of General Physiology·Yu Zhou, Christopher J Lingle
Aug 26, 2015·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Francisco J MoreraCarlos Gonzalez
Apr 30, 2016·The Journal of Biological Chemistry·Miklós LengyelPéter Enyedi
Nov 3, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jiping ZhaoHongwei Qin
Mar 17, 2017·Basic & Clinical Pharmacology & Toxicology·Christina B Falk-PetersenPetrine Wellendorph
Jul 25, 2017·Journal of Neuroinflammation·Biqiong ZhangWeizu Li
Oct 14, 2017·International Reviews of Immunology·Mallenahally Kusha VidyaRaghavendra Bhatta
Jan 23, 2018·Frontiers in Immunology·Jürg HamacherUz Stammberger
Feb 22, 2018·American Journal of Physiology. Cell Physiology·Ming LuToby C Chai
May 12, 2018·Pflügers Archiv : European journal of physiology·Alex M DopicoJonathan H Jaggar
Feb 17, 2019·International Journal of Molecular Sciences·Zuzana NovaAndrea Calkovska

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Methods Mentioned

BETA
PCR
Assay
ELISA
nuclear translocation

Software Mentioned

GraphPad
Ingenuity Pathway Analysis ( Qiagen )

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