Black Raspberries and Protocatechuic Acid Mitigate DNFB-Induced Contact Hypersensitivity by Down-Regulating Dendritic Cell Activation and Inhibiting Mediators of Effector Responses.

Nutrients
Kelvin AndersonSteve Oghumu

Abstract

Contact hypersensitivity (CHS) is the most common occupational dermatological disease. Dendritic cells (DCs) mediate the sensitization stage of CHS, while T-cells facilitate the effector mechanisms that drive CHS. Black raspberry (Rubus occidentalis, BRB) and BRB phytochemicals possess immunomodulatory properties, but their dietary effects on CHS are unknown. We examined the effects of diets containing BRB and protocatechuic acid (PCA, a constituent of BRB and an anthocyanin metabolite produced largely by gut microbes), on CHS, using a model induced by 2,4-dinitrofluorobenze (DNFB). Mice were fed control diet or diets supplemented with BRB or PCA. In vitro bone-marrow derived DCs and RAW264.7 macrophages were treated with BRB extract and PCA. Mice fed BRB or PCA supplemented diets displayed decreased DNFB-induced ear swelling, marked by decreased splenic DC accumulation. BRB extract diminished DC maturation associated with reduced Cd80 expression and Interleukin (IL)-12 secretion, and PCA reduced IL-12. Dietary supplementation with BRB and PCA induced differential decreases in IL-12-driven CHS mediators, including Interferon (IFN)-γ and IL-17 production by T-cells. BRB extracts and PCA directly attenuated CHS-promoting macropha...Continue Reading

References

Apr 1, 1996·Journal of Leukocyte Biology·G Trinchieri, F Gerosa
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hitoshi AkibaJean-François Nicolas
Feb 21, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·George MillerRonald P Dematteo
Dec 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Veronica Athie-MoralesCatharien M U Hilkens
Jun 21, 2005·Bioorganic & Medicinal Chemistry·Katrin Keppler, Hans-Ulrich Humpf
Oct 31, 2007·The Journal of Experimental Medicine·Miyako NagamachiShuh Narumiya
Dec 21, 2007·Clinical Reviews in Allergy & Immunology·Yoshinori Sasaki, Setsuya Aiba
Jun 12, 2008·Carcinogenesis·Gary David StonerBruce Cordell Casto
Dec 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Curtis J HenryElizabeth M Hiltbold
Jan 14, 2009·Cancer Prevention Research·Li-Shu WangGary D Stoner
Jun 26, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Donggou HeHui Xu
Oct 21, 2009·Allergy·M VocansonJ-F Nicolas
Feb 26, 2010·The Journal of Investigative Dermatology·Asako OgawaShinichi Sato
Jun 12, 2010·Immunological Reviews·Silke PaustUlrich H von Andrian
Oct 5, 2010·Clinical & Developmental Immunology·Mingli XuTakayuki Yoshimoto
Nov 26, 2010·Carcinogenesis·David C MontroseDaniel W Rosenberg
May 21, 2011·Bioscience, Biotechnology, and Biochemistry·Shota SakaiTakashi Hirata
Aug 31, 2012·The Journal of Investigative Dermatology·Tetsuya HondaKenji Kabashima
Jan 30, 2013·International Immunopharmacology·Miaomiao WeiXuming Deng
Mar 2, 2013·Allergy, Asthma & Immunology Research·Ju-Hee SeoSoo-Jong Hong
Sep 12, 2013·Clinical & Developmental Immunology·Matthias Peiser
Sep 26, 2013·The Journal of Investigative Dermatology·Shu Zhen ChongDavid M Kemeny
Apr 3, 2016·Current Protocols in Immunology·Anthony A GaspariStefan F Martin
Feb 22, 2017·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A GrefA Bergström
May 10, 2017·Journal of Oral and Maxillofacial Pathology : JOMFP·Rohan PulgaonkarZama Moosvi
Apr 26, 2018·Journal of Immunology Research·Dimitra KelepouriLazaros I Sakkas

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
PCA
PMA
ELISA
PCR
flow cytometry

Software Mentioned

FlowJo
GraphPad Prism
IDT RealTime qPCR Tool
Leica Application Suite
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved