blaCTX-M-15 carried by IncF-type plasmids is the dominant ESBL gene in Escherichia coli and Klebsiella pneumoniae at a hospital in Ghana

Diagnostic Microbiology and Infectious Disease
Alex AgyekumJonathan R Iredell

Abstract

Escherichia coli and Klebsiella pneumoniae producing extended-spectrum β-lactamases (ESBLs) are among the most multidrug-resistant pathogens in hospitals and are spreading worldwide. Horizontal gene transfer and spread of high-risk clones are involved in ESBL dissemination. Investigation of the resistance phenotypes of 101 consecutive clinical E. coli (n=58) and K. pneumoniae (n=43) isolated at the Komfo Anokye Teaching Hospital in Ghana over 3 months revealed 63 (62%) with an ESBL phenotype. All 63 had a blaCTX-M gene, and sequence analysis showed that 62 of these were blaCTX-M-15. blaCTX-M-15 was linked to ISEcp1 and orf477Δ in all isolates, and most isolates also carried blaTEM, aac(3)-II, aacA4cr, and/or blaOXA-30 genes on IncF plasmids. XbaI/pulsed-field electrophoresis showed heterogeneity among isolates of both species, suggesting that blaCTX-M-15 dissemination is caused by horizontal gene transfer rather than clonal spread of these species in Ghana.

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Citations

May 6, 2016·Annals of Clinical Microbiology and Antimicrobials·Daniel Oduro-MensahSammy Tawiah Sackey
May 26, 2016·International Journal of Medical Microbiology : IJMM·Daniel EibachJürgen May
Jul 1, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carola VenturiniJonathan R Iredell
May 10, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Alireza JafariHadi Sedigh Ebrahim-Saraie
Oct 2, 2019·Water Research·Richard L CarneyJustin R Seymour
Mar 3, 2021·The ISME Journal·William MatlockUNKNOWN REHAB consortium
May 7, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Annicet-Clotaire DikoumbaSylvain Godreuil

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