Bladder cancer cells interact with vascular endothelial cells triggering EGFR signals to promote tumor progression

International Journal of Oncology
Zhixin HuangJinhai Fan

Abstract

Although important progress has been made in elucidating the role of the tumor microenvironment in the development of bladder cancer, little is currently known regarding the interactions with vascular endothelial cells (ECs) that promote cancer progression. In the present study, it is reported that epidermal growth factor receptor ligands induced by the upregulation of vascular endothelial growth factor (VEGF)‑A and VEGF‑C via the VEGF receptor (R)2/nuclear factor‑κB signaling pathway in ECs, may trigger EGFR signaling in bladder cancer cells and promote bladder cancer progression. Furthermore, the interaction between bladder cancer cells and ECs enhanced EC recruitment though the CXCL1/CXCL5/CXCL8‑CXCR2 pathway. Western blotting was used to evaluate the presence of VEGFR, EGFR and nuclear factor‑κB, and reverse transcription‑quantitative polymerase chain reaction was used to evaluate the expression of VEGFR ligands and EGFR ligands. The present results indicate the mechanism by which the indirect interplay between bladder cancer cells and vascular ECs promotes cancer progression, through the VEGFR2 signaling pathway in vascular ECs and through the EGFR signaling pathway in bladder cancer cells.

References

Nov 10, 1995·The Journal of Biological Chemistry·R M StrieterD Marriott
Jun 24, 2000·Annals of the New York Academy of Sciences·Y SatoM Shibuya
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C L AddisonR M Strieter
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 4, 2002·Nature Cell Biology·Eitan Shaulian, Michael Karin
Jul 2, 2002·American Journal of Respiratory Cell and Molecular Biology·Audrey RichterDonna E Davies
Sep 5, 2002·Nature Reviews. Immunology·Ann Richmond
Jan 8, 2003·Seminars in Oncology·Judah Folkman
Apr 20, 2004·Critical Reviews in Oncology/hematology·Amar BennasrounePierre Hubert
May 12, 2004·Nature Reviews. Drug Discovery·Napoleone FerraraWilliam Novotny
Jul 5, 2005·Urology·P H RajjayabunJ K Mellon
Feb 20, 2008·Neoplasia : an International Journal for Oncology Research·Kristy A WarnerJacques E Nör
Jul 28, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Christina Barbara Ching, Donna Elizabeth Hansel
Nov 25, 2011·Carcinogenesis·W Andrew YeudallSumitra Deb
Jan 22, 2013·Pathology, Research and Practice·Sezgin GunesHasan Bagci
Jul 19, 2013·F1000prime Reports·Zachary L Smith, Thomas J Guzzo
Oct 15, 2013·International Journal of Cancer. Journal International Du Cancer·Julien CalderaroYves Allory
Jan 24, 2014·European Urology·Saurabh ChavanAhmedin Jemal
Dec 30, 2014·Carcinogenesis·Neelam MukherjeeRita Ghosh
Jun 25, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Yuan-Feng TianZhang-Qun Ye
Dec 5, 2015·Frontiers in Pharmacology·Aline Appert-CollinAmar Bennasroune

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Citations

Dec 19, 2019·Frontiers in Genetics·Victor G MartinezJesus Maria Paramio

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Methods Mentioned

BETA
PCR
light microscopy

Software Mentioned

GraphPad
GraphPad Prism

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