PMID: 8583579Mar 1, 1996Paper

Bladder injury model induced in rats by exposure to protamine sulfate followed by bacterial endotoxin

The Journal of Urology
P C SteinC L Parsons

Abstract

A bladder injury model was developed using protamine sulfate (PS) and endotoxin lipopolysaccharide (LPS) administered intravesically to female Sprague-Dawley rats. Experimental and control animals were catheterized and intravesically exposed to PS-LPS, PS, LPS, or phosphate buffered saline. After 4, 24 or 72 hours, rats were sacrificed. Urines and bladder tissues were then obtained. Bladder mucosal permeability was evaluated by measuring 14C-urea uptake 24 hours after injury. Repeated instillations of PS/LPS were also made in another group of rats over a period of 5 weeks to attempt to establish a more serious mucosal injury, possibly reflected by altered staining of the collagen IV component of the urinary basement membrane (UBM). Histological examination of the tissues indicated a maximal inflammatory response in the mucosa 4 hours after instillation of PS/LPS. Neutrophils and macrophages in close proximity to the UBM and intraepithelially could be demonstrated. Bladder permeability was significantly altered (26.9% 14C-urea uptake) in rats assayed 24 hours after the PS/LPS treatment, but not after exposure to PS or LPS alone (11.9 and 17.5%, respectively). Protease activity detected in urines from experimental, but not contro...Continue Reading

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Citations

Jan 1, 1997·Urological Research·J Luber-NarodR E Carraway
Oct 10, 2008·American Journal of Physiology. Renal Physiology·Mary Beth Klinger, Margaret A Vizzard
Apr 17, 2014·BioMed Research International·Eric J GonzalezMargaret A Vizzard
Mar 1, 2017·Physiological Reports·Koji DogishiShuji Kaneko
Aug 24, 2011·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Shin Young LeeMoo Yeol Lee
Jul 14, 2000·The Journal of Urology·D R EricksonV P Bhavanandan
Feb 26, 2008·The Journal of Urology·W BoucherT C Theoharides
Mar 17, 2004·The Journal of Urology·Katherine L Meyer-Siegler, Pedro L Vera
Sep 4, 2012·Magyar sebészet·Péter JáromiPetra Hartmann
Nov 23, 2010·BJU International·Shinsuke OkadaKenjiro Kohri
Jun 11, 2011·Neurourology and Urodynamics·Dale E BjorlingWade Bushman

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