Bladder smooth muscle cell phenotypic changes and implication of expression of contractile proteins (especially caldesmon) in rats after partial outlet obstruction

International Journal of Urology : Official Journal of the Japanese Urological Association
Seiji MatsumotoTakashi Kurita

Abstract

The purpose of the present study was to investigate morphological changes in bladder smooth muscle of rats with partial outlet obstruction. We investigated smooth muscle cell phenotypic changes and implication of synthetic phenotype in contractility decrease and bladder compliance after bladder outlet obstruction. Partial bladder outlet obstruction was introduced in female rats. Bladder were removed at 1, 3, 6, 10 and 20 weeks after the obstruction. Temporal pattern of changes in bladder mass, light microscopic pathogenesis and phenotypic expression of the bladder smooth muscle cells in the electron micrographs were investigated. Expression of contractile protein was also investigated by the immunoblotting method. Marked increase in bladder mass with marked thickening of smooth muscle layer was observed at 1 week after obstruction. The ratio of myocytes exhibiting contractile and synthetic phenotypes was almost constant until 6 weeks after the obstruction, but thereafter, synthetic phenotypes gradually increased and the ratio (synthetic/contractile phenotype) was 1.5-fold at 20 weeks after the obstruction. Caldesmon was most markedly expressed after the obstruction among contractile proteins examined by the immunoblotting metho...Continue Reading

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Citations

Mar 9, 2011·Biomechanics and Modeling in Mechanobiology·Rebecca L HeiseMichael S Sacks
Dec 22, 2009·The Journal of Urology·Victor May, Margaret A Vizzard
Jan 29, 2008·Pharmacology & Therapeutics·Martin C Michel, Maurits M Barendrecht
Dec 17, 2008·BJU International·Seiji MatsumotoRobert Levin
Jul 28, 2011·Journal of Cellular Physiology·Roger M IlaganJohn W Ludlow
Dec 28, 2017·Investigative and Clinical Urology·Naoki Aizawa, Yasuhiko Igawa
Jun 17, 2006·Journal of Applied Physiology·John E SpeichPaul H Ratz

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