PMID: 8611760Dec 1, 1995Paper

Blasticidin S deaminase gene (BSD): a new selection marker gene for transformation of Arabidopsis thaliana and Nicotiana tabacum

Bioscience, Biotechnology, and Biochemistry
K TamuraIsamu Yamaguchi

Abstract

Arabidopsis thaliana and Nicotiana tabacum were transformed to blasticidin S (BS) resistance with BSD (the BS deaminase gene from Aspergillus terreus) using the Agrobacterium-mediated transformation method. Expression of BSD allowed direct selection of transformants by the fungicide, and both kinds of transgenic plants showed high level of resistance phenotype at 100 ppm of BS sprayed on the leaves. Using Botrytis cinerea, the causal fungus of gray mold disease, it was exemplified that application of BS could control the disease in transgenic tobacco with negligible phytotoxicity.

Citations

Jan 10, 2002·Biochemical and Biophysical Research Communications·Makoto KimuraIsamu Yamaguchi
Nov 8, 2005·Journal of Biotechnology·Tetsuko Ochiai-FukudaMakoto Kimura
Jan 22, 2002·Metabolic Engineering·Philip A LessardAnthony J Sinskey
Jun 3, 2021·The New Phytologist·Eftychios FrangedakisKeiko Sakakibara

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