Blastocoele collapse by micropipetting prior to vitrification gives excellent survival and pregnancy outcomes for human day 5 and 6 expanded blastocysts

Human Reproduction
Kenichiro HiraokaKazuo Kinutani

Abstract

Manual puncture of the trophectoderm of human blastocysts with a needle before vitrification increases their survival rate, but the embryos take a long time to re-expand. This study examined whether causing human blastocysts to collapse by manual pipetting before vitrification would allow more rapid re-expansion and improve pregnancy rates. After embryo transfer in IVF cycles, surplus embryos that developed to the expanded blastocyst stage were placed in cryoprotectant and then artificially shrunk by mechanical pipetting with a fine hand-drawn glass pipette slightly smaller in diameter than the blastocyst. The shrunken embryos were placed in a small volume of vitrification solution and plunged into liquid nitrogen on a cryotop. The blastocysts were thawed by warming and then dilution in 1 mol/l sucrose. Of 49 expanded vitrified blastocysts, 48 (98%) re-expanded within 3 h after warming. Following transfer (48 blastocysts in 28 cycles), 14 women (50%) became clinically pregnant, and the implantation rate was 33% (16/48). Eight healthy babies have been born in six deliveries, and the other eight pregnancies are ongoing. To date, there have been no spontaneous abortions. The results suggest that artificial shrinkage with pipetting...Continue Reading

References

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Citations

Feb 10, 2006·Journal of Assisted Reproduction and Genetics·So-Young LeeJin-Ho Lim
Jun 11, 2009·Journal of Assisted Reproduction and Genetics·Mojtaba Rezazadeh ValojerdiBahar Movaghar
Oct 16, 2009·Journal of Assisted Reproduction and Genetics·Kenichiro HiraokaKazuo Kinutani
Dec 8, 2009·Journal of Assisted Reproduction and Genetics·Junqiang ZhangGuo Qing Tong
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Dec 15, 2010·Journal of Assisted Reproduction and Genetics·Hiroshi IwayamaMasanori Yamashita
Jun 12, 2012·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Xinling RenHanwang Zhang
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