Blastocyst-induced changes in the bovine endometrial transcriptome

Reproduction : the Official Journal of the Society for the Study of Fertility
C PassaroP Lonergan

Abstract

The objectives of this study were (i) to determine whether blastocyst-induced responses in endometrial explants were detectable after 6- or 24-h co-culture in vitro; (ii) to test if direct contact is required between embryos and the endometrial surface in order to stimulate endometrial gene expression; (iii) to establish the number of blastocysts required to elicit a detectable endometrial response; (iv) to investigate if upregulation of five interferon-stimulated genes (ISGs) in the endometrium was specific to the blastocyst stage and (v) to test if alterations in endometrial gene expression can be induced by blastocyst-conditioned medium. Exposure of endometrial explants to Day 8 blastocysts in vitro for 6 or 24 h induced the expression of ISGs (MX1, MX2, OAS1, ISG15, RSAD2); expression of IFNAR1, IFNAR2, NFKB1, IL1B, STAT1, LGALS3BP, LGALS9, HPGD, PTGES, ITGB1, AKR1C4, AMD1 and AQP4 was not affected. Culture of explants in the presence of more than five blastocysts was sufficient to induce the effect, with maximum expression of ISGs occurring in the presence of 20 blastocysts. This effect was exclusive to blastocyst stage embryos; oocytes, 2-cell embryos or Day 5 morulae did not alter the relative abundance of any of the tra...Continue Reading

References

Sep 1, 1992·Biology of Reproduction·J J Hernandez-LedezmaR M Roberts
Aug 1, 1990·Biology of Reproduction·C E FarinR M Roberts
Apr 1, 1983·Biology of Reproduction·G D BallN L First
Mar 10, 1998·Molecular Reproduction and Development·H M KubischR M Roberts
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·M A LarsonR M Roberts
Jul 12, 2002·Molecular Reproduction and Development·Dimitrios RizosPatrick Lonergan
Jul 27, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·C A GrayT E Spencer
Sep 13, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·P LonerganM P Boland
Dec 25, 2003·Molecular Reproduction and Development·Koji KimuraR Michael Roberts
Nov 18, 2005·Developmental Biology·Séverine A DegrelleIsabelle Hue
Feb 16, 2006·Reproduction, Fertility, and Development·Natalie I AlexopoulosAlan O Trounson
Jun 27, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·M ClementeP Lonergan
Dec 17, 2009·Reproduction, Fertility, and Development·Roberto SartoriMilo C Wiltbank
Apr 22, 2011·Biology of Reproduction·M ClementeD Rizos
Dec 14, 2011·Journal of Proteome Research·Marta MuñozEnrique Gómez
Feb 14, 2012·American Journal of Reproductive Immunology : AJRI·Alan Maia BorgesIain Martin Sheldon
Aug 24, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Thomas E SpencerPatrick Lonergan
Mar 25, 2014·Molecular Reproduction and Development·Roney dos Santos RamosMario Binelli
Apr 29, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·E Gómez, M Muñoz
May 1, 2015·Biology of Reproduction·Veronica MailloDimitrios Rizos
Nov 27, 2015·Biological Reviews of the Cambridge Philosophical Society·Eline WydoogheAnn Van Soom
May 11, 2016·Theriogenology·Alireza Fazeli, William V Holt
Apr 27, 2017·Reproduction, Fertility, and Development·Milena Lopes OliveiraMario Binelli
Jul 28, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Fuller W Bazer, William W Thatcher
Sep 10, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·N Forde, P Lonergan
Oct 7, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Thomas R HansenThomas E Spencer
Dec 11, 2017·Theriogenology·E GómezM Muñoz

❮ Previous
Next ❯

Citations

Oct 31, 2018·American Journal of Reproductive Immunology : AJRI·Anup K TalukderAkio Miyamoto
Apr 13, 2019·Biology of Reproduction·José María SánchezPat Lonergan
Jun 9, 2019·Scientific Reports·Mariana SponchiadoMario Binelli
Jan 1, 2018·Reproduction, Fertility, and Development·P LonerganTrudee Fair
Apr 25, 2020·International Journal of Molecular Sciences·Irene Malo EstepaNiamh Forde
Jun 2, 2020·Frontiers in Cell and Developmental Biology·Yentel Mateo-OteroBeatriz Fernandez-Fuertes
Jan 25, 2020·Theriogenology·Beatriz Rodríguez-AlonsoDimitrios Rizos
Jan 30, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Keerthie DissanayakeAlireza Fazeli
Mar 26, 2021·American Journal of Reproductive Immunology : AJRI·Laura FernándezRosanna Ramhorst

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.