Blebbing of Dictyostelium cells in response to chemoattractant.

Experimental Cell Research
Paul D Langridge, Robert R Kay

Abstract

Stimulation of Dictyostelium cells with a high uniform concentration of the chemoattractant cyclic-AMP induces a series of morphological changes, including cell rounding and subsequent extension of pseudopodia in random directions. Here we report that cyclic-AMP also elicits blebs and analyse their mechanism of formation. The surface area and volume of cells remain constant during blebbing indicating that blebs form by the redistribution of cytoplasm and plasma membrane rather than the exocytosis of internal membrane coupled to a swelling of the cell. Blebbing occurs immediately after a rapid rise and fall in submembraneous F-actin, but the blebs themselves contain little F-actin as they expand. A mutant with a partially inactivated Arp2/3 complex has a greatly reduced rise in F-actin content, yet shows a large increase in blebbing. This suggests that bleb formation is not enhanced by the preceding actin dynamics, but is actually inhibited by them. In contrast, cells that lack myosin-II completely fail to bleb. We conclude that bleb expansion is likely to be driven by hydrostatic pressure produced by cortical contraction involving myosin-II. As blebs are induced by chemoattractant, we speculate that hydrostatic pressure is one ...Continue Reading

References

Jun 1, 1978·The Journal of Cell Biology·B S Eckert, E Lazarides
Mar 4, 1976·Nature·M S Bretscher
Nov 1, 1989·Cell Regulation·J A Spudich
Jul 1, 1988·Developmental Biology·D WesselsJ Spudich
Feb 19, 1988·Science·D Bray, J G White
Sep 1, 1970·The Biochemical Journal·D J Watts, J M Ashworth
Aug 30, 1983·Biochemical and Biophysical Research Communications·S J McRobbie, P C Newell
Jun 1, 1995·The Journal of Cell Biology·C C Cunningham
Oct 1, 1993·The Journal of Cell Biology·L W Janson, D L Taylor
Jan 1, 1996·Cell Motility and the Cytoskeleton·M YanaiS M Kelly
Mar 14, 1998·The Journal of Cell Biology·J C MillsR N Pittman
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y FukuiS Inoué
May 23, 2001·Current Biology : CB·P A SteimleT T Egelhoff
Feb 26, 2003·Cell·Thomas D Pollard, Gary G Borisy
Mar 26, 2003·The Journal of Cell Biology·Jean-Pierre LevraudPierre Golstein
Jun 11, 2003·Eukaryotic Cell·Eunkyung LeeDavid A Knecht
Jul 20, 2004·Cell Motility and the Cytoskeleton·Yukako AsanoTaro Q P Uyeda
Nov 6, 2004·FEBS Letters·Markus FischerAnnette Müller-Taubenberger
May 20, 2005·Nature·Guillaume T CharrasT J Mitchison

❮ Previous
Next ❯

Citations

Mar 19, 2014·The Journal of Cell Biology·Effie BastounisJuan C Lasheras
Mar 13, 2014·The Journal of Cell Biology·Evgeny ZatulovskiyRobert R Kay
Jul 31, 2014·Proceedings of the National Academy of Sciences of the United States of America·Richard A TysonTill Bretschneider
Jun 19, 2008·PloS One·Emmanuel DeriveryAlexis Gautreau
Oct 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jean-Yves TinevezEwa Paluch
Dec 4, 2009·Molecular Biology of the Cell·Ruedi MeiliJuan C Lasheras
Sep 3, 2010·Journal of Cell Science·Roberto ZanchiRobert R Kay
Apr 10, 2010·Journal of Experimental Botany·Laura Zonia
Jan 22, 2008·Nature Cell Biology·Sophie Pinner, Erik Sahai
Jan 11, 2012·The Journal of Cell Biology·Julia K GildenMatthew F Krummel
Jun 11, 2008·The Journal of Cell Biology·Oliver T Fackler, Robert Grosse
Nov 29, 2011·Trends in Cell Biology·Romain Levayer, Thomas Lecuit
Apr 26, 2011·Trends in Plant Science·Laura Zonia, Teun Munnik
Nov 20, 2010·European Journal of Cell Biology·Leann NormanHelim Aranda-Espinoza
Feb 19, 2015·Biophysical Journal·Begoña Álvarez-GonzálezJuan C Del Álamo
Sep 8, 2009·Trends in Cell Biology·Jason S King, Robert H Insall
May 19, 2009·Trends in Plant Science·Laura Zonia, Teun Munnik
Nov 23, 2007·Current Opinion in Microbiology·Robert Insall, Natalie Andrew
Jun 8, 2007·Experimental Cell Research·Paul D Langridge, Robert R Kay
Feb 13, 2007·Trends in Plant Science·Laura Zonia, Teun Munnik
Aug 11, 2006·The EMBO Journal·Dirk Dormann, Cornelis J Weijer
Aug 30, 2008·Journal of Microscopy·S Pinner, E Sahai
Aug 30, 2008·Journal of Microscopy·G T Charras
Dec 6, 2008·Biology of the Cell·Robert J Cain, Anne J Ridley
Jun 22, 2013·Current Opinion in Cell Biology·Ewa K Paluch, Erez Raz
Jul 21, 2015·Cell Adhesion & Migration·Claudia Tanja Mierke
Jul 31, 2010·Cell Adhesion & Migration·Matthew R Clay, Mary C Halloran
Feb 4, 2014·Current Biology : CB·Louise FetsRobert R Kay
Apr 3, 2007·Current Biology : CB·Kathryn M EisenmannArthur S Alberts
Nov 9, 2010·Seminars in Cell & Developmental Biology·Markus Maniak
Sep 23, 2010·Biophysical Journal·Leann L NormanHelim Aranda-Espinoza
Dec 12, 2012·PloS One·Daisuke TakaoShigenori Nonaka
Oct 6, 2016·PloS One·Markela IboZachary R Gagnon
Jul 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Martin BergertEwa Paluch
Jan 14, 2017·Molecular Oncology·Pahini PandyaVictoria Sanz-Moreno
Oct 5, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Yuan Xiong, Pablo A Iglesias
Jul 17, 2008·Nature Reviews. Molecular Cell Biology·Guillaume Charras, Ewa Paluch
May 27, 2008·Nature Reviews. Molecular Cell Biology·Robert R KayOliver Hoeller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinosarcoma

Carcinosarcoma is a malignant neoplasm that contains elements of carcinoma and sarcoma so extensively intermixed as to indicate neoplasia of epithelial and mesenchymal tissue. Discover the latest research on carcinosarcoma here.

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.