Jun 1, 1993

Bleomycin affects cell wall anchorage of mannoproteins in Saccharomyces cerevisiae

Antimicrobial Agents and Chemotherapy
R BeaudouinP N Lipke

Abstract

Bleomycin induces strand breakage in DNA through disruption of glycosidic linkages. We investigated the ability of bleomycin to damage yeast cell walls, which are composed primarily of carbohydrate. Bleomycin treatment of intact yeast cells facilitated enzymatic conversion of yeasts to spheroplasts. Bleomycin treatment also altered anchorage of mannoproteins to the cell wall matrix in intact cells or isolated cell walls. Cell surface mannoproteins were labelled with 125I, and their solubilization was monitored. Seventeen hour treatments with bleomycin released some of the label directly into treatment supernatants and facilitated extraction of mannoproteins by dithiothreitol and lytic enzymes. Bleomycin treatments as short as 10 min caused changes in extraction of mannoproteins from intact cells. Specifically, cell wall anchorage of several mannoproteins was affected by the drug. There were drug-induced changes in extractability of mannoproteins with apparent molecular weights of 96,000, 80,000, 61,000, 41,000, 31,500, and 21,000 (determined after deglycosylation with endo-N-acetylglucosaminidase H). The similarity of results obtained in the presence and absence of cycloheximide, the appearance of cell wall effects after only 1...Continue Reading

Mentioned in this Paper

Mannoproteins
Cell Wall
Spheroplasts
Histiocytosis With Joint Contractures and Sensorineural Deafness
Cell Adhesion
Yeast Proteins
Membrane Glycoproteins
Saccharomyces cerevisiae

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