Abstract
Recent advances in chemotherapy have focused on the benefit of high dose regimens, increasing the dose intensity of conventional chemotherapy. However, unacceptable cytotoxicity and genotoxicity on normal cells often impairs the proper management of patients. Phosphoaminothiol WR-1065, the active metabolite of amifostine, appears to protect normal cells and tissues against cytotoxic exposure to radiation or chemotherapeutic agents. Nevertheless, there is disagreement in findings on amifostine protection against bleomycin-induced severe side effects which have suggested that amifostine effectiveness against bleomycin-induced genotoxicity in normal leukocytes and tumour line cells K562 be studied. DNA damage was detected by single cell gel electrophoresis (or Comet) assay, a technique able to detect DNA strand breaks, alkali-labile sites and incomplete excision repair events in individual cells and which appears to be an ideal tool for assessing variability in response of different cell types in vitro. WR-2721 appears to selectively protect healthy leukocytes but not K562 tumoral cells. On the other hand, data on the inter- and intra-individual sensitivity to bleomycin and amifostine suggest that individual metabolic/genetic diff...Continue Reading
References
Mar 1, 1991·Mutation Research·L F Povirk, M J Austin
Dec 1, 1991·Pigment Cell Research·B D ThrallG G Meadows
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Aug 1, 1986·International Journal of Radiation Oncology, Biology, Physics·A RussoJ B Mitchell
Aug 3, 1994·Biochemical Pharmacology·R W Byrnes, D H Petering
Jul 1, 1993·Mutation Research·V J McKelvey-MartinA Collins
Feb 1, 1995·Mutation Research·D W FairbairnK L O'Neill
Dec 10, 1995·Molecular & General Genetics : MGG·G R HoffmannL G Littlefield
Aug 17, 1996·Mutation Research·L F Povirk
Dec 9, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C W TaylorR L Capizzi
Apr 29, 1998·Leukemia·L MattiiM Petrini
Oct 29, 1998·British Journal of Cancer·L PierelliS Mancuso
Oct 8, 1999·Toxicology Letters·S M Rappaport, K Yeowell-O'Connell
Feb 9, 2000·Cancer Chemotherapy and Pharmacology·F MarzaticoL Castagna
Sep 20, 2000·Leukemia·A BuschiniC Rossi
Citations
Apr 25, 2012·Archives of Toxicology·Supriya SwarnkarChandishwar Nath
Feb 9, 2005·Cancer Chemotherapy and Pharmacology·Luciano VellónIrene Larripa
Sep 16, 2003·Mutation Research·P PoliT M A D Zucchi
Dec 17, 2008·Environmental Health Perspectives·Veronika A EhrlichSiegfried Knasmüller
Aug 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Peggy RegulusJean-Luc Ravanat
Aug 1, 2012·Neurochemical Research·Supriya SwarnkarChandishwar Nath
Nov 28, 2014·Molecular Neurobiology·Poonam GoswamiSarika Singh
Sep 17, 2011·Mutation Research·Sarika SinghSharad Sharma
Aug 29, 2009·Mutation Research·N AydemirR Bilaloglu
Apr 13, 2007·Mutation Research·George R HoffmannChristine J Willett
Oct 7, 2008·Basic & Clinical Pharmacology & Toxicology·Su Jin KangHai Won Chung
Apr 29, 2009·Environmental Toxicology·Eliseo AlmeidaMontserrat Llagostera
Feb 13, 2007·Biochemical Pharmacology·Annamaria BuschiniPaola Poli
Mar 16, 2016·Journal of Medicinal Chemistry·Michal HoferŠtefan Galbavý
Jun 27, 2015·Environmental Toxicology and Pharmacology·Poonam GoswamiSarika Singh
Mar 3, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Sonam GuptaSarika Singh
May 24, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Michal HoferMartin Falk
Oct 25, 2016·Toxicology Mechanisms and Methods·Nasrin Ghassemi-BarghiAbbas Jafarian-Dehkordi
Sep 17, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ricardo M CameloMiriam T P Lopes
Dec 21, 2006·Journal of Applied Toxicology : JAT·Abhinav JagetiaShalini Jha
Jan 24, 2018·Neurotoxicology·Dinesh Kumar VermaSarika Singh