Bleomycin-kanamycin resistance as a marker of the presence of transposon Tn5 in clinical strains of Escherichia coli.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
F BaqueroR Gómez-Lus

Abstract

The aminoglycoside modifying enzyme aminoglycoside 3'-phosphotransferase II (APH(3')II) is encoded for on transposon Tn5 by the aphA gene, in the same operon as the ble gene determining bleomycin resistance. To document this linkage 82 kanamycin-resistant Escherichia coli strains of clinical origin were studied; all 18 isolates presenting bleomycin-kanamycin resistance were shown by an enzymatic assay to produce APH(3')II, and the presence of Tn5 was demonstrated by gene hybridization. Similarly, bleomycin-kanamycin resistance was shown to be linked to APH(3')II production in Salmonella spp. The epidemiology of strains with Tn5-encoded APH(3')II may thus be studied, at least in Escherichia coli, by a simple diffusion test using bleomycin and kanamycin discs.

References

Jan 1, 1975·Methods in Enzymology·M J Haas, J E Dowding
Jan 1, 1979·Molecular & General Genetics : MGG·R A JorgensenW S Reznikoff
Nov 1, 1979·Antimicrobial Agents and Chemotherapy·M H Perlin, S A Lerner
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D E BergJ D Rochaix
Feb 1, 1986·Antimicrobial Agents and Chemotherapy·M H Perlin, S A Lerner
Mar 28, 1988·FEBS Letters·A GatignolG Tiraby
Jul 1, 1985·Journal of Bacteriology·J L San MillanF Moreno
Sep 1, 1986·Molecular & General Genetics : MGG·P MazodierF Gasser
Dec 1, 1985·European Journal of Clinical Microbiology·M LaRocco, A Robinson
Sep 1, 1981·Antimicrobial Agents and Chemotherapy·G P Bongaerts, G M Kaptijn

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