BliI, a restriction endonuclease from Bacillus licheniformis

FEBS Letters
P L ManachiniL Benazzi

Abstract

From Bacillus licheniformis a site-specific restriction endonuclease, named BliI, has been purified and characterized. BliI was able to digest lambda DNA at pH 9.1 over a wide temperature range (25-65 degrees C). Digestion of lambda and psi X174 DNAs with BliI produced banding patterns identical to those seen with HaeIII. Therefore, BliI and HaeIII endonculeases are isoschizomers.

References

Feb 1, 1977·Journal of Bacteriology·J F Catterall, N E Welker
Mar 23, 1968·Nature·M Meselson, R Yuan
Jan 1, 1980·Methods in Enzymology·V Pirrotta, T A Bickle

Related Concepts

BecAII endonuclease
DNA Restriction Enzymes
DNA, Viral
Substrate Specificity
Type II site-specific deoxyribonuclease

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