PMID: 6166093Mar 1, 1981Paper

Block staining of mammalian tissues with hematoxylin and eosin

Stain Technology
I F Hine

Abstract

Various mammalian tissues were stained en bloc with hematoxylin and eosin after fixation and prior to embedding in paraffin wax and sectioning. The choice of fixative is important and best results are obtained using Worcester's Fluid, a combination of saturated aqueous mercuric chloride, formaldehyde, and glacial acetic acid. After fixation, blocks of tissue up to 1.5 cm thick are stained for seven days in hematoxylin. Excess stain is removed by washing tissues in running water overnight. Tissue blocks then are dehydrated with graded concentrations of ethyl alcohols to 80% and counterstained, with further dehydration, in 0.5% spirit soluble eosin in 90% ethyl alcohol for five days. The tissue is subsequently transferred to 90% ethyl alcohol overnight to differentiate eosin staining; dehydration is completed in absolute ethyl alcohol. The blocks are cleared in in cedarwood oil and briefly in xylene prior to embedding, sectioning, and mounting. Following removal of wax by xylene, coverslips are applied. General morphological and histological features were particularly well differentiated and very selectively and reliably stained by this method.

References

Apr 1, 1951·Stain Technology·R C PEARLMAN, B C COLE

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Citations

Jan 1, 1985·Stain Technology·S A Kincaid, S A Evander
May 29, 2009·Micron : the International Research and Review Journal for Microscopy·Jin Ik Lim, Yong-Keun Lee
May 21, 2009·Microscopy Research and Technique·Jin Ik Lim, Yong-Keun Lee
Jan 12, 2010·Microscopy Research and Technique·Jin Ik LimYong-Keun Lee
Mar 6, 2017·Molecular Medicine Reports·Wei ZhengZhaowei Meng
Oct 31, 2020·Drug Development and Industrial Pharmacy·Kalaiyarasan VijayakumarArun Meyyazhagan
Mar 1, 2020·Archives of Medical Research·Chandrasekaran Sankaranarayanan, Karunanithi Kalaivani
Sep 15, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Qihan LiShaohui Ma

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