Blockade by nanomolar resveratrol of quantal catecholamine release in chromaffin cells

Molecular Pharmacology
José C Fernández-MoralesAntonio M G de Diego

Abstract

The cardiovascular protecting effects of resveratrol, an antioxidant polyphenol present in grapes and wine, have been attributed to its vasorelaxing effects and to its anti-inflammatory, antioxidant, and antiplatelet actions. Inhibition of adrenal catecholamine release has also been recently implicated in its cardioprotecting effects. Here, we have studied the effects of nanomolar concentrations of resveratrol on quantal single-vesicle catecholamine release in isolated bovine adrenal chromaffin cells. We have found that 30 to 300 nM concentrations of resveratrol blocked the acetylcholine (ACh) and high K(+)-evoked quantal catecholamine release, amperometrically measured with a carbon fiber microelectrode. At these concentrations, resveratrol did not affect the whole-cell inward currents through nicotinic receptors or voltage-dependent sodium and calcium channels, neither the ACh- or K(+)-elicited transients of cytosolic Ca(2+). Blockade by nanomolar resveratrol of secretion in ionomycin- or digitonin-treated cells suggests an intracellular site of action beyond Ca(2+)-dependent exocytotic steps. The fact that nanomolar resveratrol augmented cGMP is consistent with the view that resveratrol could be blocking the quantal secretio...Continue Reading

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Citations

Apr 20, 2011·Analytical Chemistry·Yuqing LinAndrew G Ewing
Jun 29, 2011·Molecular Nutrition & Food Research·Jacobo ElíesManuel Campos-Toimil
Apr 14, 2012·European Journal of Pharmacology·Juan Fernando PadínAntonio G García
Jan 3, 2012·Cell Calcium·Antonio G GarcíaJavier García-Sancho
Apr 19, 2013·American Journal of Physiology. Cell Physiology·Juan-Fernando PadínAntonio G García
Feb 23, 2020·BioMed Research International·Michał WicińskiMaciej Słupski

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