Blockade of a mitochondrial cationic channel by an addressing peptide: an electrophysiological study.

The Journal of Membrane Biology
J P HenryM Thieffry

Abstract

A voltage-dependent cationic channel of large conductance is observed in phospholipid bilayers formed at the tip of microelectrodes from proteoliposomes derived from mitochondrial membranes. This channel was blocked by a 13-residue peptide with the sequence of the amino terminal extremity of the nuclear-coded subunit IV of cytochrome c oxidase. The blockade was reversible, voltage- and dose-dependent. The peptide did not affect the activity of a Torpedo chloride channel observed under the same conditions. From experiments with phospholipid monolayers, it is unlikely that the peptide inserts into bilayers under the experimental conditions used. The blockade was observed from both sides of the membrane, being characterized by more frequent transitions to the lower conductance states, and a maximum effect was observed around 0 mV. Channels, the gating mechanism of which had been eliminated by exposure to trypsin, were also blocked by the peptide. For trypsinized channels, the duration of the closure decreased and the blockade saturated at potentials below -30 mV. These observations are consistent with a translocation of the peptide through the channel. Dynorphin B, which has the same length and charge as the peptide, had some bloc...Continue Reading

References

Jan 18, 1989·Biochimica Et Biophysica Acta·F U HartlW Neupert
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D S Allison, G Schatz
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A Baker, G Schatz
Jun 9, 1988·Biochimica Et Biophysica Acta·G von Heijne
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S J SingerM P Yaffe
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D H HochL L Simpson
Aug 1, 1983·Biophysical Journal·R Coronado, R Latorre
Jun 15, 1982·European Journal of Biochemistry·M SchleyerW Neupert
Jan 1, 1981·Physiological Reviews·R Latorre, O Alvarez
Jul 1, 1983·The Journal of General Physiology·W Hanke, C Miller
Jan 27, 1984·Biochimica Et Biophysica Acta·R HayS Gasser
Jan 5, 1983·Biochimica Et Biophysica Acta·W HankeG Boheim

❮ Previous
Next ❯

Citations

Oct 1, 1994·Journal of Bioenergetics and Biomembranes·M Zoratti, I Szabó
Feb 1, 1993·Journal of Bioenergetics and Biomembranes·F FèvreM Thieffry
Feb 1, 1992·Journal of Bioenergetics and Biomembranes·C A MannellaJ Dias
Feb 1, 1992·Journal of Bioenergetics and Biomembranes·K W KinnallyD B Zorov
Apr 1, 1996·Journal of Bioenergetics and Biomembranes·J P HenryM Thieffry
Apr 1, 1996·Journal of Bioenergetics and Biomembranes·G BathoriM Zoratti
Jul 17, 1995·Biochimica Et Biophysica Acta·M Zoratti, I Szabò
Jul 19, 2008·Annual Review of Analytical Chemistry·John J KasianowiczVincent M Stanford
Mar 24, 2007·Annual Review of Biochemistry·Stefan Wennmalm, Sanford M Simon
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·J J KasianowiczD W Deamer
Oct 24, 2007·Biophysical Journal·Debabrata Panja, Gerard T Barkema
May 6, 2008·Biophysical Journal·Melissa PoynorStephan Nussberger
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·M C Sorgato, O Moran
Feb 26, 1991·European Journal of Biochemistry·J F ChichJ P Henry
Apr 17, 2010·Small·William R HesseMinjun Kim
Apr 3, 2014·Physiological Reviews·Ildiko Szabo, Mario Zoratti
Jul 7, 1995·The Journal of Biological Chemistry·T A Lohret, K W Kinnally
Jun 29, 2006·Physical Review Letters·Joanne Klein WolterinkDebabrata Panja
May 6, 2016·Journal of Bioenergetics and Biomembranes·María Luisa CampoSonia Martínez-Caballero
May 23, 1997·The Journal of Biological Chemistry·Y Lu, A D Beavis
Jul 16, 2013·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Mehrnaz AnvariM Reza Rahimi Tabar
Apr 12, 1995·Biochimica Et Biophysica Acta·I SzabóM Zoratti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.