Blockade of electron transport during ischemia protects cardiac mitochondria.

The Journal of Biological Chemistry
Edward J LesnefskyCharles L Hoppel

Abstract

Subsarcolemmal mitochondria sustain progressive damage during myocardial ischemia. Ischemia decreases the content of the mitochondrial phospholipid cardiolipin accompanied by a decrease in cytochrome c content and a diminished rate of oxidation through cytochrome oxidase. We propose that during ischemia mitochondria produce reactive oxygen species at sites in the electron transport chain proximal to cytochrome oxidase that contribute to the ischemic damage. Isolated, perfused rabbit hearts were treated with rotenone, an irreversible inhibitor of complex I in the proximal electron transport chain, immediately before ischemia. Rotenone pretreatment preserved the contents of cardiolipin and cytochrome c measured after 45 min of ischemia. The rate of oxidation through cytochrome oxidase also was improved in rotenone-treated hearts. Inhibition of the electron transport chain during ischemia lessens damage to mitochondria. Rotenone treatment of isolated subsarcolemmal mitochondria decreased the production of reactive oxygen species during the oxidation of complex I substrates. Thus, the limitation of electron flow during ischemia preserves cardiolipin content, cytochrome c content, and the rate of oxidation through cytochrome oxidase...Continue Reading

References

Dec 27, 1977·Biochemistry·S B Vik, R A Capaldi
Mar 26, 1992·Biochimica Et Biophysica Acta·F L Hoch
Aug 15, 1991·Archives of Biochemistry and Biophysics·N L ParinandiH H Schmid
Jan 1, 1991·Journal of Molecular and Cellular Cardiology·W FlamengH Van Belle
Dec 29, 1989·Biochemical and Biophysical Research Communications·D K DasP S Rao
Sep 1, 1985·Journal of Molecular and Cellular Cardiology·H M PiperP G Spieckermann
Apr 1, 1984·The Biochemical Journal·B Halliwell, J M Gutteridge
May 1, 1984·Journal of Molecular and Cellular Cardiology·G K Asimakis, V R Conti
Nov 15, 1993·Archives of Biochemistry and Biophysics·G ParadiesE Quagliariello
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G AnthonyB Kadenbach
Apr 1, 1993·Journal of Bioenergetics and Biomembranes·G T Babcock, C Varotsis
Feb 15, 1993·Biochemical and Biophysical Research Communications·M Degli EspostiG Lenaz
Jun 1, 1997·Journal of Bioenergetics and Biomembranes·Z Salamon, G Tollin
Sep 23, 1997·Journal of Molecular and Cellular Cardiology·T L Vanden HoekL B Becker
Aug 17, 1999·Current Opinion in Structural Biology·N Grigorieff
Oct 26, 1999·Biochemical and Biophysical Research Communications·Y ShidojiK Yagi
Sep 12, 2000·Free Radical Biology & Medicine·S RahaB H Robinson
May 22, 2001·Archives of Biochemistry and Biophysics·L Gille, H Nohl
Jul 11, 2001·Journal of Molecular and Cellular Cardiology·E J LesnefskyC L Hoppel
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Martin OttSten Orrenius
Sep 19, 2002·The Journal of Biological Chemistry·Julie St-PierreMartin D Brand
Dec 17, 2002·The Journal of Biological Chemistry·Derick HanEnrique Cadenas
Apr 23, 2003·FEBS Letters·Vilmante Borutaite, Guy C Brown
May 15, 2003·Archives of Biochemistry and Biophysics·Shadi MoghaddasEdward J Lesnefsky
Jul 4, 2003·The Journal of Biological Chemistry·Qun ChenEdward J Lesnefsky

❮ Previous
Next ❯

Citations

Dec 1, 2008·Journal of Cardiovascular Translational Research·Sabu Thomas, Edward O McFalls
Jun 15, 2007·Cardiovascular Research·Cameron DezfulianMark T Gladwin
Jun 23, 2009·Nature Reviews. Neuroscience·Lorenzo GalluzziGuido Kroemer
Feb 23, 2007·The New England Journal of Medicine·J Travis HinsonChristine E Seidman
Aug 8, 2007·The Journal of Experimental Medicine·Sruti ShivaMark T Gladwin
Dec 7, 2007·Antioxidants & Redox Signaling·Lindsay S Burwell, Paul S Brookes
Sep 9, 2008·Antioxidants & Redox Signaling·John J MieyalMelissa D Shelton
Dec 17, 2009·Antioxidants & Redox Signaling·Amadou K S CamaraDavid F Stowe
Dec 8, 2011·Antioxidants & Redox Signaling·Diane E Handy, Joseph Loscalzo
Feb 20, 2013·Antioxidants & Redox Signaling·Natalia GorenkovaAlexander Galkin
Oct 21, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Graham M StrubSarah Spiegel
Mar 12, 2009·Journal of Cardiovascular Pharmacology·Genevieve C Sparagna, Edward J Lesnefsky
Dec 9, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Radha AnanthakrishnanRavichandran Ramasamy
Apr 9, 2008·Physiological Reviews·Elizabeth Murphy, Charles Steenbergen
Oct 16, 2012·Circulation Research·Andrew M WaltersPaul S Brookes
Jan 1, 2012·Journal of Experimental Stroke & Translational Medicine·Shimin Liu, Jiang-Fan Chen
Jul 30, 2010·Cardiovascular Research·Mariana G Rosca, Charles L Hoppel
Feb 8, 2014·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Gina L J Galli, Jeffrey G Richards
Nov 10, 2013·European Journal of Pharmacology·Sunanda PandaVilayanoor Ramamurthy
Feb 5, 2008·Free Radical Biology & Medicine·Annalisa Del PreteMichele Lorusso
Mar 23, 2007·Analytical and Bioanalytical Chemistry·P KaewsuyaD Ekhterae
Sep 6, 2012·Heart Failure Reviews·Mariana G Rosca, Charles L Hoppel
Jan 19, 2016·Cell Metabolism·Edward T ChouchaniMichael P Murphy
Jan 19, 2016·Biochimica Et Biophysica Acta·Stefan DröseAlexander Galkin
Jun 16, 2012·Translational Research : the Journal of Laboratory and Clinical Medicine·Christine Tanaka-EspositoEdward J Lesnefsky
Jun 10, 2010·Biochemical and Biophysical Research Communications·Qun ChenEdward J Lesnefsky
Oct 1, 2009·Nitric Oxide : Biology and Chemistry·Sruti Shiva
Dec 4, 2015·Toxicology and Applied Pharmacology·Haiyan HeZhelong Xu
Mar 24, 2009·Journal of Molecular and Cellular Cardiology·Edward J LesnefskyCharles L Hoppel

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.