Blockade of neuronal nitric oxide synthase abolishes the toxic effects of Tx2-5, a lethal Phoneutria nigriventer spider toxin

Toxicon : Official Journal of the International Society on Toxinology
C M YonamineM A P Camillo

Abstract

The primary goal of this study was to determine whether Tx2-5, a sodium channel selective toxin obtained from the venom of the spider Phoneutria nigriventer, produced penile erection by means of nitric oxide mechanism. Toxin identity was analyzed by MALDI-TOF, ES-MS and N-terminal amino acid sequencing. Pretreating mice with the non-selective nitric oxide synthase (NOS) inhibitor N(omega)-Nitro-L-arginine methyl ester hydrochloride (L-NAME) and the selective neuronal-NOS inhibitor 7-Nitroindazole (7-NI) prior to Tx2-5 i.p. (10 microg/25 g mouse) injection challenged the hypothesis above. Controls were injected with the D-isomer or DMSO or saline. Results demonstrated that L-NAME inhibited penile erections in about half the animals treated, while 7-NI completely abolished this effect. Interestingly 7-NI also abolished all the other symptoms of intoxication induced by Tx2-5, including salivation, respiratory distress and death. Tx2-5 killed all the animals of the control group and no one in the 7-NI-treated group. We conclude that (1) intraperitoneal injections of Tx2-5 induce a toxic syndrome that include penile erection, hypersalivation and death by respiratory distress or pulmonary edema; (2) pretreatment with the non-selectiv...Continue Reading

References

Jan 1, 1992·Journal of Toxicology. Clinical Toxicology·M GueronS Sofer
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·L Rezende JúniorC R Diniz
Mar 1, 1995·Biology of Reproduction·A L Burnett
Jan 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·S G FigueiredoM Richardson
Nov 1, 1994·Neuropharmacology·A Argiolas
Nov 1, 1993·Toxicon : Official Journal of the International Society on Toxinology·E AntunesG de Nucci
Feb 1, 1993·Naunyn-Schmiedeberg's Archives of Pharmacology·D A AraújoP S Beirão
Oct 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·E RegoG De Nucci
Aug 1, 1997·Progress in Neuro-psychopharmacology & Biological Psychiatry·M R Melis, A Argiolas
Jan 1, 2000·Lancet·T BurdettF Slater
Apr 1, 2000·Revista Do Instituto De Medicina Tropical De São Paulo·F BucaretchiR J Vieira
Jan 10, 2001·Current Opinion in Urology·F Giuliano, O Rampin
Mar 17, 2001·Clinical and Experimental Pharmacology & Physiology·R P LeeH I Chen
Sep 24, 2002·Pharmacology & Therapeutics·B J Nevin, K J Broadley
Jan 29, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cleber E TeixeiraGilberto De Nucci
Jun 21, 2011·Toxicon : Official Journal of the International Society on Toxinology·Lanfranco R P TronconeJosé N Nobrega

❮ Previous
Next ❯

Citations

Dec 4, 2008·Critical Care Medicine·Perenlei EnkhbaatarDaniel L Traber
Sep 13, 2008·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Fábio BucaretchiStephen Hyslop
Aug 29, 2012·The Journal of Sexual Medicine·Kenia P NunesMaria Elena de Lima
Apr 27, 2010·Toxicon : Official Journal of the International Society on Toxinology·F S TorresM R V Diniz
Jun 16, 2009·Toxicon : Official Journal of the International Society on Toxinology·Fabiola E VillanovaMiguel Srougi
Apr 1, 2008·Nitric Oxide : Biology and Chemistry·Shilpa Kumar-RoinéSerge Pauillac
Apr 10, 2008·Toxicon : Official Journal of the International Society on Toxinology·K P NunesM E De Lima
Jun 26, 2007·Toxicon : Official Journal of the International Society on Toxinology·Carina T RizziLanfranco R P Troncone
May 15, 2007·Brain Research Bulletin·Maria Alice da Cruz-HöflingCatarina Rapôso
Jun 21, 2011·Toxicon : Official Journal of the International Society on Toxinology·Lanfranco R P TronconeJosé N Nobrega
Nov 10, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·M RichardsonM N Cordeiro
Apr 16, 2013·Toxicon : Official Journal of the International Society on Toxinology·Kenia P NunesMaria E De Lima
Jul 4, 2012·Toxicon : Official Journal of the International Society on Toxinology·Kátia R M LeiteLanfranco R P Troncone
May 13, 2020·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Ana Luiza Bittencourt PaivaMarcelo Ribeiro Vasconcelos Diniz
Mar 26, 2021·PLoS Neglected Tropical Diseases·Subramanian SenthilkumaranSakthivel Vaiyapuri

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.