Abstract
Nanomolar concentrations of atropine have been considered up to now to be selective for blockade of muscarinic receptors for acetylcholine. A collateral finding indicated to us that these low concentrations of atropine could also target the neuronal nicotinic receptors. We report here a detailed study on this novel property of atropine. Catecholamine release, measured on-line with amperometry in chromaffin cells stimulated with acetylcholine pulses was blocked by atropine in a competitive manner. To corroborate a direct action of atropine on nicotinic receptors, we have employed N,N-dimethyl-N'-phenyl-piperazinium (DMPP), a pure nicotinic receptor agonist; atropine blocked its secretory responses with an IC50 of 2.04 nM. Nicotinic currents, recorded with the whole cell configuration of the patch-clamp technique were blocked by atropine in a concentration-dependent manner (IC50 of 11 nM), also showing a competitive nature. Nicotinic receptor currents in oocytes expressing bovine alpha7 and alpha3beta4 nicotinic receptors were blocked by atropine with an IC50 of 11.2 and 46.8 nM, respectively. Atropine (30 nM) also decreased the increment of the cytosolic calcium concentrations after stimulation with 30 microM DMPP in bovine chro...Continue Reading
References
Apr 1, 1991·The Journal of Membrane Biology·P H Barry, J W Lynch
Jan 1, 1991·General Pharmacology·D B McKayL J Wallace
Jan 29, 1990·Brain Research·J SadoshimaN Akaike
Mar 1, 1990·Analytical Biochemistry·M A MoroA G García
Aug 1, 1989·The Journal of Physiology·R Miledi, I Parker
Sep 25, 1984·Nucleic Acids Research·P A Krieg, D A Melton
Apr 15, 1981·Biochemical Pharmacology·G DeromeS Lemaire
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Dec 1, 1983·Neuroscience·V CeñaA G Garcia
Dec 15, 1983·Biochemical Pharmacology·A M SwilemN Azila
Nov 1, 1982·British Journal of Pharmacology·S M KirpekarM T Schiavone
Nov 18, 1994·Cell·A B ElgoyhenS Heinemann
Nov 16, 1994·Biochemical Pharmacology·H HouchiM Oka
Nov 21, 1998·The European Journal of Neuroscience·J G BarbaraK Takeda
Mar 26, 1999·Cell Calcium·E Neher
Sep 16, 2000·The Journal of Physiology·S N Sudweeks, J L Yakel
Oct 2, 2001·The Journal of Physiology·L MaggiE Cherubini
Nov 1, 1957·The Journal of Pharmacy and Pharmacology·G D LEACH
Dec 16, 1961·Nature·W W DOUGLAS, R P RUBIN
Nov 1, 1961·The Journal of Physiology·W W DOUGLAS, R P RUBIN
Mar 17, 2004·Archives of Biochemistry and Biophysics·O L ZaikaE A Lukyanetz
Jul 3, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jorge FuentealbaAntonio G García
May 4, 1936·The Journal of Physiology·H H DaleM Vogt
Citations
Jul 21, 2011·British Journal of Anaesthesia·M KoxP Pickkers
Jun 23, 2011·Learning & Memory·Chris J TinsleyE Clea Warburton
Feb 27, 2008·Acta Pharmacologica Sinica·Qiang LiuJie Wu
Feb 22, 2013·Cerebral Cortex·J C GonzálezJ M Hernández-Guijo
Mar 4, 2008·The European Journal of Neuroscience·J J Arnaiz-CotJ M Hernández-Guijo
Sep 22, 2007·Journal of Neurochemistry·Alberto Pérez-Alvarez, Almudena Albillos
Dec 15, 2010·Brain Research·Gang Q Li, Manning J Correia
Jul 15, 2018·The Journal of Pharmacology and Experimental Therapeutics·Carmen NanclaresAntonio G García
Nov 7, 2019·Journal of Neurochemistry·Trevor W Stone
Feb 14, 2007·ChemMedChem·M Novella RomanelliFulvio Gualtieri
Aug 10, 2017·Pflügers Archiv : European journal of physiology·Manuel Criado