Abstract
There is great interest in any new discoveries in malaria vaccine research. Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) shows promise in this area and may be used together with other merozoite antigens as a potential vaccine. In the present study, a bioinformatics prediction approach was applied to a PfRH5 B-cell epitope, and two B-cell epitope distributions were selected. Antibodies against the two PfRH5 distributions were obtained and the growth activity inhibition was measured. No inhibition of the P. falciparum CY strain was found, but the growth of the P. falciparum 3D7 strain was inhibited by all of the antibodies, in contrast to the results of other studies. It was additionally found that certain quantities of protein led to the inhibition of the parasitic invasion. Equally noteworthy was that the survival time of the group immunized with a portion of PfRH5 was significantly longer than that of the group immunized with the full-length protein, following infection by P. berghei ANKA. The present study produced conflicting results in in vitro and in vivo experiments, although the accuracy of the evaluation may be lessened due to the use of a murine malaria model. The findings of the present study m...Continue Reading
References
Mar 25, 1978·Journal of Molecular Biology·J GarnierB Robson
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·J C RaynerJ W Barnwell
Mar 13, 2002·Experimental Parasitology·L CuiJ Williams
Sep 14, 2002·Infection and Immunity·Helen M TaylorAnthony A Holder
Feb 28, 2003·The EMBO Journal·Manoj T DuraisinghAlan F Cowman
Dec 23, 2004·Molecular Microbiology·Tony TrigliaAlan F Cowman
Jul 30, 2005·Cell·Niraj H ToliaLeemor Joshua-Tor
Aug 20, 2005·The Journal of Parasitology·William Trager, James B Jensen
Aug 27, 2005·Science·Janine StubbsAlan F Cowman
Jun 30, 2006·Vaccine·Jun MiaoLiwang Cui
Jul 14, 2006·Molecular and Biochemical Parasitology·Susana NeryDavid J Conway
Jul 16, 2008·Cell Host & Microbe·Karen HaytonThomas E Wellems
Oct 2, 2008·PloS One·Marilis RodriguezCheryl A Lobo
Nov 13, 2008·International Journal for Parasitology·Jake BaumAlan F Cowman
Dec 15, 2010·Infection and Immunity·Sash LopatickiAlan F Cowman
Oct 20, 2011·The New England Journal of Medicine·UNKNOWN RTS,S Clinical Trials PartnershipPreeti Vansadia
Nov 15, 2011·Nature·Cécile CrosnierGavin J Wright
Dec 22, 2011·Nature Communications·Alexander D DouglasSimon J Draper
May 19, 2012·Pathogens and Global Health·Tony Nolan
Jul 24, 2012·Malaria Journal·Prajakta S JogdandMichael Theisen
Aug 14, 2012·Expert Opinion on Therapeutic Targets·Takashi Muramatsu
Nov 1, 2012·Malaria Journal·Elizabeth VillasisSara Lustigman
Nov 3, 2012·PloS One·Mary Lopez-PerezSara Lustigman
Nov 13, 2012·PLoS Pathogens·Andrew R WilliamsSimon J Draper
Nov 14, 2012·Vaccine·Leyla Y BustamanteJulian C Rayner