Blockade of the effects of TGF-beta1 on mesangial cells by overexpression of Smad7.

Journal of the American Society of Nephrology : JASN
Ruihua ChenRichard V Paul

Abstract

Smad7, a protein induced by transforming growth factor-beta1 (TGF-beta1) in many target cells, inhibits TGF-beta1 signal transduction and is thought to mediate an intracellular negative feedback response that limits TGF-beta1 effects. It is possible that overexpression of Smad7 could block specified effects of TGF-beta1 on mesangial cells, a TGF-beta target in glomerular disease. Smad7 mRNA was induced by TGF-beta1 within 1 h in a concentration-dependent manner in a transformed mouse mesangial cell (MMC) line. Uptake of (14)C-spermidine from the medium by MMC and the transcriptional activity of a segment of the human collagen pro-alpha2 type 1 chain (COL1A2) promoter fused to a luciferase reporter gene were used as indices of TGF-beta1. Treatment with TGF-beta1 increased (14)C-spermidine uptake rate in a time-, concentration-, and temperature-dependent manner. For example, exposure to 1 ng/ml TGF-beta1 for 15 h increased uptake approximately twofold, a response that was attenuated by cycloheximide. Transfection of Smad7 expression vector into MMC abrogated both TGF-beta1-dependent stimulation of spermidine uptake and COL1A2 promoter activity. It is concluded that: (1) TGF-beta1 induces Smad7 in MMC; (2) (14)C-spermidine uptake ...Continue Reading

References

Mar 1, 1997·The Journal of International Medical Research·Y TaniguchiM Yamakido
May 1, 1997·Kidney International·W A Border, N A Noble
May 9, 1998·The Journal of Biological Chemistry·L WangK Sharma
Aug 26, 1998·Biochemical and Biophysical Research Communications·M AfrakhteP ten Dijke
Oct 6, 1998·Annual Review of Biochemistry·J Massagué
Jul 7, 1999·The Journal of Clinical Investigation·A NakaoI Iwamoto
May 3, 2000·The Journal of Cell Biology·L ChoyR Derynck

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Citations

Mar 26, 2002·Journal of the American Society of Nephrology : JASN·H William SchnaperAnne-Christine Poncelet
Dec 31, 2002·Journal of the American Society of Nephrology : JASN·Claire C Sharpe, Bruce M Hendry
Apr 23, 2003·Journal of the American Society of Nephrology : JASN·Roger M Mason, Nadia Abdel Wahab
May 23, 2003·Journal of the American Society of Nephrology : JASN·Hui Y LanRichard J Johnson
Apr 24, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jin H LiHui Y Lan
Apr 20, 2004·Current Opinion in Nephrology and Hypertension·Nadia Abdel Wahab, Roger M Mason
Dec 6, 2003·American Journal of Physiology. Renal Physiology·P VrljicakI R Gupta
May 25, 2004·International Journal of Experimental Pathology·Kathleen C Flanders
Jun 14, 2005·Experimental Cell Research·Nadia Abdel WahabRoger M Mason
Nov 11, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Marta Ruiz-OrtegaJesús Egido
Sep 16, 2005·Transplant International : Official Journal of the European Society for Organ Transplantation·Debra A HullettBryan N Becker
Jan 20, 2006·Liver International : Official Journal of the International Association for the Study of the Liver·Xingjun LiuJames Q Yin
Sep 14, 2007·Kidney International·J NieX Q Yu
Aug 21, 2009·World Journal of Pediatrics : WJP·Wei WuShu-Mei Chen
Aug 29, 2009·Cancer Letters·John A CookJames B Mitchell
Jul 2, 2011·Journal of the American Society of Nephrology : JASN·Christiane Rüster, Gunter Wolf
Nov 23, 2011·Cell and Tissue Research·Francisco J López-Hernández, Jose M López-Novoa
Mar 15, 2012·Nature Communications·Alfiya AkhmetshinaJörg H W Distler

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