Blockade of the release of the neuropeptide leucokinin to determine its possible functions in fly behavior: chemoreception assays

Peptides
Begoña López-AriasPilar Herrero

Abstract

Previous studies have revealed leucokinin (LK) expression in the brain and ventral ganglion of Drosophila CNS. One pair of protocerebrum neurons located in the lateral horn area (LHLK) surrounds the peduncles of the mushroom bodies while two pairs of subesophageal neurons (SELKs) project extended processes to the tritocerebrum and through a cervical connection to the ventral ganglion. There, axons of eight or nine pairs of abdominal (ABLK) neurons leave the CNS through the abdominal nerves and processes connecting each other ipsilaterally and contralaterally. The neural functions of LK remain largely unknown, especially those related to Drosophila behavior. Here, we have studied the role of LK in olfactory and gustatory perception by keeping the LK neurons electrically silent through targeted expression of inward rectifier K(+) channels. In order to examine the effects of LK failure, we first analyzed the dehydration response, comparing the leucokinin-silent individuals with their parents as a control. Our results showed significant differences that demonstrate the effectiveness of the method. We then tested the olfactory behavioral response to a set of odorants over a range of concentrations in a T-maze paradigm in which flies...Continue Reading

References

Sep 1, 1985·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·T Tully, W G Quinn
Apr 1, 1994·Cell and Tissue Research·Y ChenH H Hagedorn
May 1, 1997·Journal of Neurobiology·R F StockerJ S de Belle
Jan 24, 2003·The Journal of Comparative Neurology·Pilar HerreroInmaculada Canal
Jun 5, 2003·Advances in Genetics·Paul H Taghert, Jan A Veenstra
Mar 27, 2004·Current Biology : CB·Nobuaki K TanakaKei Ito
Jun 19, 2004·Current Biology : CB·Natasha ThorneHubert Amrein
Sep 18, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Guillaume IsabelPhilippe Rosay
Aug 27, 2005·PLoS Biology·Christoph Melcher, Michael J Pankratz
Nov 18, 2005·Molecular and Cellular Neurosciences·Asa M E WintherAlberto Ferrús
Jan 13, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Haojiang LuanBenjamin H White
Jun 9, 2006·Cell and Tissue Research·Dick R Nässel, Uwe Homberg
Apr 25, 2007·Nature Reviews. Neuroscience·Alex C Keene, Scott Waddell

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Citations

Oct 2, 2012·Peptides·Pilar Herrero
Oct 25, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Pilar HerreroFernando J Diaz-Benjumea
Oct 16, 2015·Journal of the American Society for Mass Spectrometry·Jelle CaersMatthias B Van Hiel
Jun 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hyeogsun KwonPatricia V Pietrantonio
Nov 5, 2016·Annual Review of Entomology·Liliane SchoofsMatthias Boris Van Hiel
May 26, 2020·Cellular and Molecular Life Sciences : CMLS·Takashi KoyamaKim Rewitz
Feb 2, 2018·Proceedings of the National Academy of Sciences of the United States of America·Do-Hyoung KimMichael E Adams
Feb 7, 2021·International Journal of Molecular Sciences·Dick R Nässel, Shun-Fan Wu
Mar 7, 2021·International Journal of Molecular Sciences·Dick R Nässel
May 2, 2013·Development·Alicia Estacio-GómezFernando J Díaz-Benjumea

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