Blockade of Toll-Like Receptor 4 Attenuates Erectile Dysfunction in Diabetic Rats

The Journal of Sexual Medicine
K P NunesR C Webb

Abstract

While increased toll-like receptor (TLR)4 activity may contribute to the pathophysiology of vascular diseases, the molecular mechanisms disrupted by this receptor in the vasculature are still poorly understood. Additionally, it is unknown if TLR4 mediates erectile dysfunction (ED) during diabetes. To investigate whether pharmacological blockade of TLR4 affects erectile function in a murine model of diabetes. Sprague Dawley rats (Charles River Laboratory, Wilmington, MA, USA) received a single streptozotocin injection (65 mg/kg, 28 days) and were treated with an anti-TLR4 antibody (1 μg/d, intraperitoneally) for the last 14 days of the treatment. Additionally, cavernosal strips were acutely incubated for 30 minutes with CLI-095 (10-5 mol/L), a TLR4 inhibitor. Functional studies, Western blotting, erectile function, immunohistochemistry, and biochemical analyses were performed. Oxidative stress, cyclic guanosine monophosphate (cGMP) levels, and functional studies were evaluated in treated and nontreated cavernosal strips from control and diabetic animals. Additionally, in vivo erectile function was assessed. Enhanced TLR4 expression was observed in corpus cavernosum from diabetic rats compared with control animals. Long-term bloc...Continue Reading

Citations

Jan 22, 2019·Hypertension·Cameron G McCarthyR Clinton Webb
Aug 22, 2018·Current Drug Targets·Amanda Almeida de OliveiraKenia Pedrosa Nunes
Sep 1, 2020·American Journal of Hypertension·Amanda Almeida de Oliveira, Kenia Pedrosa Nunes
Jun 30, 2019·International Journal of Molecular Sciences·Amanda Almeida de OliveiraKenia Pedrosa Nunes
Mar 21, 2021·The Journal of Sexual Medicine·Fernanda PrivieroR Clinton Webb
Sep 25, 2020·Small GTPases·Kenia Pedrosa Nunes, R Clinton Webb
Apr 6, 2021·Diabetes & Metabolism Journal·Wang-Soo Lee, Jaetaek Kim
Nov 7, 2020·Archives of Physiology and Biochemistry·Hongyu Fu, Huiqiang Liu
May 30, 2021·Vascular Pharmacology·Amanda Almeida de Oliveira, Kenia Pedrosa Nunes

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