Blockade of Wnt-5A/frizzled 5 signaling inhibits rheumatoid synoviocyte activation

Arthritis and Rheumatism
Malini SenDennis A Carson

Abstract

It is not understood why cultured fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA) often display a persistently activated phenotype, despite removal from an inflammatory environment. Previously, we found that these FLS expressed high levels of both Wnt-5A and Frizzled 5 (Fz5), a receptor-ligand pair implicated in both limb bud and bone marrow stem cell development. The objective of the present experiments was to determine whether Wnt-5A/FzS signaling contributes to FLS activation. Wnt-5A expression in FLS was inhibited by transfection with both antisense and dominant negative (dn) vectors. Fz5 signaling was blocked with an antibody to the extracellular domain of the receptor. The effects of these treatments on the expression of the proinflammatory cytokines interleukin-6 (IL-6) and IL-15 and on the expression of receptor activator of nuclear factor kappaB ligand (RANKL) were assessed by reverse transcriptase-polymerase chain reaction and immunoblotting. Both antisense Wnt-5A and dnWnt-5A vectors, but not empty vector, diminished IL-6 and IL-15 expression in RA FLS. Anti-Fz5 antibody exerted similar effects and also reduced RANKL expression. Wnt-5A/Fz5 signaling may contribute to the activated stat...Continue Reading

References

Sep 1, 1994·Molecular and Cellular Biology·G T WongA P McMahon
Oct 1, 1996·Seminars in Arthritis and Rheumatism·R Badolato, J J Oppenheim
Apr 1, 1997·Trends in Genetics : TIG·R T MoonM Torres
Dec 6, 1997·Biochemical and Biophysical Research Communications·H TakayanagiY Koshihara
Feb 7, 1998·Genes & Development·K M Cadigan, R Nusse
May 23, 1998·Annual Review of Immunology·S GhoshE B Kopp
Jun 17, 1998·Arthritis and Rheumatism·A E Koch
Jan 19, 1999·Annual Review of Cell and Developmental Biology·A Wodarz, R Nusse
Jan 26, 1999·Annals of the New York Academy of Sciences·M GiarréA M Brown
Feb 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SenD A Carson
Feb 29, 2000·Arthritis and Rheumatism·E M GravalleseS R Goldring
Jun 20, 2000·Trends in Genetics : TIG·M KühlR T Moon

❮ Previous
Next ❯

Citations

Dec 19, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Ramiro MalgorDouglas J Goetz
Aug 19, 2007·Amino Acids·G CaponeD Kanduc
May 10, 2006·Investigational New Drugs·Nico JanssensTim Perera
May 16, 2002·Current Rheumatology Reports·Ulf Müller-LadnerSteffen Gay
Aug 30, 2008·Nature Clinical Practice. Rheumatology·Georg SchettJean-Pierre David
Dec 2, 2006·Nature Reviews. Drug Discovery·Nick Barker, Hans Clevers
Mar 6, 2013·Nature Reviews. Rheumatology·Rik J LoriesNancy E Lane
Jul 13, 2005·DNA and Cell Biology·Karen E YatesRichard G Reish
Oct 13, 2006·Molecular Biology of the Cell·T Néstor H MasckauchánJan Kitajewski
May 1, 2002·Current Opinion in Rheumatology·J Alastair GracieIain B McInnes
Jun 7, 2007·Current Opinion in Rheumatology·Mark L Johnson, Mohamed A Kamel
Apr 27, 2011·Acta Physiologica·A KikuchiS Matsumoto
Aug 28, 2009·Pigment Cell & Melanoma Research·Michael P O'Connell, Ashani T Weeraratna
Jan 10, 2002·Annals of the Rheumatic Diseases·M Corr, N J Zvaifler
Oct 9, 2003·Annals of the Rheumatic Diseases·I B McInnesF Y Liew
Mar 3, 2009·American Journal of Respiratory Cell and Molecular Biology·Louis J VugaNaftali Kaminski
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Andy J Chien, Randall T Moon
Jan 19, 2013·World Journal of Orthopedics·Teruhito YamashitaNobuyuki Udagawa
Oct 23, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shi-Yie ChengChang-Hung Chou
Oct 16, 2015·Cell Biology International·Somayeh ArabzadehAmir Hassan Zarnani
Dec 17, 2015·Rheumatology·Martijn H van den BoschPeter M van der Kraan
May 13, 2008·Trends in Molecular Medicine·Sonja HermanGeorg Schett
Jun 9, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Julie R HensJohn J Wysolmerski
Jun 9, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xiaochao WeiRegis J O'Keefe
Apr 26, 2016·Journal of Immunology Research·Juan ShiXiaoming Liu
Aug 7, 2008·BMB Reports·Jang-Soo ChunMeeyoung Park
Jun 30, 2016·The New England Journal of Medicine·Pelin O Simsek KiperRoland Baron
Aug 30, 2016·Cancers·Marwa S AsemM Sharon Stack
Dec 14, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Martina RaunerLorenz C Hofbauer
Jun 21, 2012·Cell Biochemistry and Function·Katherine Ann StainesColin Farquharson
May 18, 2013·Scandinavian Journal of Immunology·C BergenfelzK Leandersson
Oct 19, 2011·Arthritis and Rheumatism·Elena KudryavtsevaVyacheslav A Adarichev
Nov 18, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Anthony D PersonJamie L Lohr
Mar 3, 2010·Immunological Reviews·Nicole C Walsh, Ellen M Gravallese
Nov 19, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Chan Soo ShinRoberto Civitelli
Sep 10, 2011·Acta Biochimica Et Biophysica Sinica·Antara De
Apr 19, 2017·Clinical and Experimental Immunology·C WehmeyerA J Naylor

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