Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress

Gastroenterology Research and Practice
Chen ZhaoBaoping Yu

Abstract

Visceral hypersensitivity is a common feature of irritable bowel syndrome. Cholinergic system involves in the development of visceral hypersensitivity, and high-affinity choline transporter (CHT1) is of crucial importance in choline uptake system. However, involvement of CHT1 in visceral hypersensitivity remains unknown. The research aimed to study the CHT1 expression in dorsal root ganglions (DRGs) and the role of CHT1 in visceral hypersensitivity. Repetitive water avoidance stress (WAS) was used to induce visceral hypersensitivity in rats. Colorectal distension (CRD) was determined, and the abdominal withdrawal reflex (AWR) and threshold intensity data were recorded to measure the visceral sensitivity. After intraperitoneal injection of hemicholinium-3 (HC-3), the specific inhibitor of CHT1, CRD data were also recorded. The CHT1 expression of DRGs was investigated by Western blotting, immunohistochemistry, and quantitative RT-PCR. Acetylcholine levels in the DRGs were detected by the assay kit. Repetitive WAS increased the AWR score of CRD at high distension pressure and decreased the mean threshold of rats. The CHT1 expression and acetylcholine concentration of DRG were significantly increased in WAS rats. After the administ...Continue Reading

References

Jan 1, 1994·Journal of Neural Transmission. Parkinson's Disease and Dementia Section·R Rodríguez-PuertasJ Pascual
Oct 12, 2000·Biochemical and Biophysical Research Communications·S ApparsundaramR D Blakely
Dec 26, 2001·Neuroscience Letters·Yasushi KobayashiTatsuya Haga
Apr 5, 2003·Neurochemical Research·Takashi Okuda, Tatsuya Haga
Mar 5, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sylvie BradesiEmeran A Mayer
Nov 10, 2005·Autonomic Neuroscience : Basic & Clinical·G GuidryB A Habecker
Feb 7, 2007·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·L A BlackshawM Schemann
May 19, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mélanie G GareauMary H Perdue
Jun 5, 2007·Neuropharmacology·Philip G Jones, John Dunlop
Dec 25, 2010·Therapeutic Advances in Gastroenterology·Gareth J SangerLesley A Houghton
Feb 8, 2011·The Journal of Pain : Official Journal of the American Pain Society·Jia-Lie LuoZhao-Xiang Bian
Apr 2, 2011·Journal of Gastroenterology and Hepatology·Motoyori KanazawaShin Fukudo
May 4, 2011·Current Gastroenterology Reports·Giovanni BarbaraRoberto Corinaldesi
Jun 7, 2011·Nature Reviews. Gastroenterology & Hepatology·QiQi Zhou, G Nicholas Verne
Sep 4, 2012·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·B BalestraG Barbara
Nov 8, 2012·The Journal of Biological Chemistry·Takashi OkudaTatsuya Haga
Jul 17, 2013·The American Journal of Gastroenterology·Parvathi A MyerUri Ladabaum
Jul 31, 2014·Journal of Biochemistry·Tatsuya Haga
Oct 30, 2014·Neuromodulation : Journal of the International Neuromodulation Society·Elliot S Krames
Feb 26, 2016·Clinical and Translational Gastroenterology·Kenneth Barshop, Kyle Staller
May 26, 2016·Regional Anesthesia and Pain Medicine·Liong LiemJeff Kramer
Jul 20, 2016·Journal of Neurogastroenterology and Motility·Mohammad H FarzaeiRoja Rahimi
May 10, 2017·Biochemical and Biophysical Research Communications·Dan LuoBaoping Yu

❮ Previous
Next ❯

Citations

Aug 8, 2021·Journal of Clinical Medicine·Mónica GrosEva Latorre

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Prism

Related Concepts

Related Feeds

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.