Blocking amino acid transporter OsAAP3 improves grain yield by promoting outgrowth buds and increasing tiller number in rice

Plant Biotechnology Journal
Kai LuZhongming Fang

Abstract

Amino acid transporters (AATs) play indispensable roles in nutrient allocation during plant development. In this study, we demonstrated that inhibiting expression of the rice amino acid transporter OsAAP3 increased grain yield due to a formation of larger numbers of tillers as a result of increased bud outgrowth. Elevated expression of OsAAP3 in transgenic plants resulted in significantly higher amino acid concentrations of Lys, Arg, His, Asp, Ala, Gln, Gly, Thr and Tyr, and inhibited bud outgrowth and rice tillering. However, RNAi of OsAAP3 decreased significantly Arg, Lys, Asp and Thr concentrations to a small extent, and thus promoted bud outgrowth, increased significantly tiller numbers and effective panicle numbers per plant, and further enhanced significantly grain yield and nitrogen use efficiency (NUE). The promoter sequences of OsAAP3 showed some divergence between Japonica and Indica rice, and expression of the gene was higher in Japonica, which produced fewer tillers than Indica. We generated knockout lines of OsAAP3 on Japonica ZH11 and KY131 using CRISPR technology and found that grain yield could be increased significantly. These results suggest that manipulation of OsAAP3 expression could be used to increase grai...Continue Reading

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Citations

Apr 13, 2020·Journal of Experimental Botany·Le LuoGuohua Xu
Jan 4, 2019·International Journal of Molecular Sciences·Le LuoGuohua Xu
Aug 23, 2019·International Journal of Molecular Sciences·Ali RazzaqFaiz Ahmad Joyia
Oct 11, 2019·International Journal of Molecular Sciences·Xiaoxi ZhenFan Xu
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Aug 6, 2020·Plants·Guangzhe YangJixing Xia
Aug 6, 2020·Plants·Xuehui YaoXiaolei Sui
Mar 6, 2019·Annual Review of Plant Biology·Kunling ChenCaixia Gao
Jan 22, 2021·Plant Cell Reports·Dragana MiladinovicZoe Hilioti
Dec 9, 2020·Journal of Integrative Plant Biology·Yufei LiJie Luo
Feb 10, 2021·Plant Cell Reports·Xin HuangChangfu Zhu
Feb 12, 2021·Plant Signaling & Behavior·Mengmeng HouYali Zhang
Mar 19, 2021·Frontiers in Plant Science·Samantha Vivia TheMechthild Tegeder
Sep 28, 2020·Journal of Biotechnology·Mujtaba Aamir BhatArif Tasleem Jan
May 1, 2021·International Journal of Molecular Sciences·Sichul Lee, Celine Masclaux-Daubresse
Apr 11, 2021·Journal of Integrative Plant Biology·Rongna WangZhongming Fang
Jun 8, 2021·Journal of Agricultural and Food Chemistry·Mariana Rocha MaximianoOctávio Luiz Franco
Jul 16, 2021·Transgenic Research·Jerlie Mhay MatresInez H Slamet-Loedin
Jul 4, 2021·The Plant Journal : for Cell and Molecular Biology·Saori OgasawaraToshihiko Hayakawa
Aug 21, 2021·Journal of Plant Physiology·Aart J E van Bel
Aug 23, 2021·Molecular Biotechnology·Dipinte GuptaRajiv Ranjan
Aug 28, 2021·International Journal of Molecular Sciences·Mengmeng HouJingguang Chen

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Methods Mentioned

BETA
transgenic
gene knockout
PCR

Software Mentioned

SPSS
ImageJ

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