Blocking CCN2 preferentially inhibits osteoclastogenesis induced by repetitive high force bone loading.

Connective Tissue Research
Mary F BarbeSteven N Popoff

Abstract

Purpose/Aim: We recently found that blocking CCN2 signaling using a monoclonal antibody (FG-3019) may be a novel therapeutic strategy for reducing overuse-induced tissue fibrosis. Since CCN2 plays roles in osteoclastogenesis, and persistent performance of a high repetition high force (HRHF) lever pulling task results in a loss in trabecular bone volume in the radius, we examined here whether blocking CCN2 signaling would reduce the early catabolic effects of performing a HRHF task for 3 weeks. Materials and Methods: Young adult, female, Sprague-Dawley rats were operantly shaped to learn to pull at high force levels, before performing the HRHF task for 3 weeks. HRHF task rats were then left untreated (HRHF Untreated), treated in task weeks 2 and 3 with a monoclonal antibody that antagonizes CCN2 (HRHF+FG-3019), or treated with an IgG (HRHF+IgG), while continuing to perform the task. Non-task control rats were left untreated. Results: In metaphyseal trabeculae of the distal radius, HRHF Untreated and HRHF-IgG rats showed increased osteoblast numbers and other indices of bone formation, compared to controls, yet decreased trabecular bone volume, increased osteoclast numbers, and increased serum CTX-1 (a serum biomarker of bone res...Continue Reading

References

Nov 11, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ann E BarrMary F Barbe
Jul 4, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tsuyoshi ShimoMasaharu Takigawa
Jun 28, 2008·The Journal of Biological Chemistry·Anna Smerdel-RamoyaErnesto Canalis
May 19, 2009·BioFactors·Iwona Cicha, Margarete Goppelt-Struebe
Sep 11, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jane M FedorczykMary F Barbe
Aug 20, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Takashi NishidaMasaharu Takigawa
Jul 16, 2011·PloS One·Sashidhar S NakerakantiMaria Trojanowska
Oct 5, 2011·Critical Reviews in Eukaryotic Gene Expression·John A ArnottSteven N Popoff
Oct 18, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Alex G LambiSteven N Popoff
Aug 2, 2013·Human Molecular Genetics·Maria Gabriela MoralesEnrique Brandan
Dec 17, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·David B Burr
Dec 18, 2014·Current Osteoporosis Reports·Keith G AvinStuart J Warden
Mar 6, 2015·Industrial Health·Mary F BarbeAnn E Barr-Gillespie
Apr 22, 2015·Journal of Cell Communication and Signaling·Hany Mohamed KhattabMasaharu Takigawa
Sep 15, 2015·Journal of Cellular Biochemistry·Yurika MuraseSatoshi Kubota
Oct 11, 2015·Bone·Marco Brotto, Lynda Bonewald
Jan 27, 2016·Journal of the Neurological Sciences·Geoffrey M BoveMary F Barbe
Jun 4, 2018·Human Molecular Genetics·David GonzalezEnrique Brandan
Jan 31, 2019·American Journal of Respiratory and Critical Care Medicine·Simon L F Walsh, Luca Richeldi
May 2, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Mary F BarbeSteven N Popoff
Apr 1, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mary F BarbeSteven N Popoff

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Citations

Dec 4, 2020·Connective Tissue Research·Sarah M Greising, Vincent M Wang

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